推荐产品
等級
for molecular biology
品質等級
形狀
powder
技術
Southern blotting: suitable
異物活動
DNase, RNase, none detected
儲存溫度
room temp
一般說明
Sigma′s neutralization buffer is suitable for Southern blotting procedures to neutralize the pH of the gel following depurination and denaturation treatment or before transferring it to membranes.
應用
Suitable for neutralizing the gel before transfer to membranes during Southern procedures.
成分
The Neutralization Buffer is composed of 1M Tris-HCl and 1.5M NaCl.
相關產品
产品编号
说明
价格
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
其他客户在看
A southern blot assay for detection of hepatitis B virus covalently closed circular DNA from cell cultures
Cai D, et al.
Methods in Molecular Biology, 151-161 (2013)
Sambrook, J., and Russell, D. W.
Molecular Cloning: A Laboratory Manual, 6-6 (2001)
Ausubel, F.M., et al.
Short Protocols in Molecular Biology (1995)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
实验方案
An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.
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