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主要文件

N6019

Sigma-Aldrich

Neutralizing Buffer

for Northern and Southern blotting, powder

别名:

Neutralizing Buffer for Blotting Techniques

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About This Item

分類程式碼代碼:
12161700
NACRES:
NA.25

一般說明

Sigma′s neutralization buffer is suitable for Southern blotting procedures to neutralize the pH of the gel following depurination and denaturation treatment or before transferring it to membranes.

應用

Suitable for neutralizing the gel before transfer to membranes during Southern procedures.

成分

The Neutralization Buffer is composed of 1M Tris-HCl and 1.5M NaCl.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

A southern blot assay for detection of hepatitis B virus covalently closed circular DNA from cell cultures
Cai D, et al.
Methods in Molecular Biology, 151-161 (2013)
Sambrook, J., and Russell, D. W.
Molecular Cloning: A Laboratory Manual, 6-6 (2001)
Ausubel, F.M., et al.
Short Protocols in Molecular Biology (1995)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)

实验方案

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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