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Merck
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主要文件

D3435

Millipore

Dey-Engley 中和肉汤

suitable for microbiology, NutriSelect® Plus

别名:

D/E中和肉汤

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About This Item

分類程式碼代碼:
41171607
NACRES:
NA.85

無菌

non-sterile

品質等級

200

形狀

powder

儲存期限

limited shelf life, expiry date on the label

製造商／商標名

NutriSelect® Plus

最終pH值

7.6±0.2 (25 °C)

應用

environmental
food and beverages
pharmaceutical
sterility testing
surface monitoring
microbiology

儲存溫度

2-8°C

適合性

nonselective for Bacillus spp.
nonselective for Escherichia coli
nonselective for Pseudomonas spp.
nonselective for Salmonella spp.
nonselective for Staphylococcus spp.
nonselective for bacteria (General Media)
nonselective for coliforms

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相关类别

一般說明

Dey-Engley中和肉汤用于消毒剂测试，其中防腐剂和消毒剂的中和对于测定杀菌活性很重要。肉汤由提供氮和碳源的色氨酸、长链氨基酸、维生素和其他必需营养素组成。葡萄糖是一种能量来源。酵母提取物也是维生素B复合物的丰富来源。亚硫酸氢钠中和醛；巯基乙酸钠中和汞；硫代硫酸钠中和碘和氯；卵磷脂中和季铵化合物；聚山梨酸酯80，一种非离子表面活性剂，中和取代酚。溴甲酚紫色是葡萄糖利用的指标。发酵葡萄糖的微生物会把培养基从紫色变成黄色。假单胞菌属的生长不会发酵葡萄糖，可通过在肉汤表面形成的膜来检测。

應用

D-E 中和肉汤在消毒实验中用于中和防腐剂和消毒剂，这对于检测细菌活性非常重要。
Dey-Engley中和培养基用于预湿润海绵拭子样本中和消毒剂对微生物生长的残留作用。Dey-Engley中和肉汤用于消毒剂测试，其中利用防腐剂和消毒剂的中和来测定杀菌活性。此外，还用于重新悬浮Vermamoeba vermiformis（哈氏虫属）囊肿，以中和过乙酸(PAA)。

生化／生理作用

Dey-Engley中和培养基用于中和广泛的防腐剂和消毒剂，包括季铵化合物、酚类、碘类、氯、汞、甲醛和戊二醛等。巯基乙酸钠、硫代硫酸钠、亚硫酸氢钠、大豆卵磷脂和聚山梨酯80均用作中和成分。从紫色到黄色的颜色变化表示生长。

成分

成分 (g/L)
酶水解酪蛋白 5.00
酵母提取物 2.50
右旋糖 10.00
硫代硫酸钠 6.00
巯基乙酸钠 1.00
亚硫酸氢钠 2.50
卵磷脂 7.00
聚山梨酸酯 80 5.00
溴甲酚紫 0.02

準備報告

将39克Dey-Engley中和培养基悬浮于1000毫升蒸馏水中。煮沸至培养基完全溶解。使用15磅压力(121°C)进行高压灭菌的方法消毒15分钟。

法律資訊

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

象形圖

Exclamation mark
GHS07

訊號詞

Warning

危險聲明

H317

危險分類

Skin Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Faceshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCG8846
BCCG1677
BCCF1318
BCCD6196
BCCC6094

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Sensitivity of Vermamoeba (Hartmannella) vermiformis cysts to conventional disinfectants and protease
Fouque E, et al.
Journal of Water and Health, 13(2), 302-310 (2015)
A 10-day vacancy period after cleaning and disinfection has no effect on the bacterial load in pig nursery units
Luyckx K, et al.
BMC Veterinary Research, 12(1), 236-236 (2016)
Comparison of competitive exclusion with classical cleaning and disinfection on bacterial load in pig nursery units
Luyckx K, et al.
BMC Veterinary Research, 12(1), 189-189 (2016)
D Joseph Sexton et al.
Infection control and hospital epidemiology, 41(10), 1219-1221 (2020-07-01)
We tested 9 disinfectants against Candida auris using the quantitative disk carrier method EPA-MB-35-00: 5 products with hydrogen peroxide or alcohol-based chemistries were effective and 4 quaternary ammonium compound-based products were not. This work supported a FIFRA Section 18 emergency
H Maertens et al.
Poultry science, 98(7), 2972-2976 (2019-04-18)
Resistance to antibiotics threatens to become a worldwide health problem. An important attributing phenomenon in this context is that pathogens can acquire antibiotic resistance genes through conjugative transfer of plasmids. To prevent bacterial infections in agricultural settings, the use of
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