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Merck
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文件

T4415

Sigma-Aldrich

Tris-硼酸-EDTA缓冲溶液

BioReagent, suitable for electrophoresis, 10× concentrate

别名:

TBE 缓冲液

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About This Item

MDL號碼:
MFCD00236358
分類程式碼代碼:
41105319
PubChem物質ID:
329826793
NACRES:
NA.25

品質等級

200

產品線

BioReagent

形狀

solution

雜質

DNase and RNase, none detected

適合性

suitable for electrophoresis

SMILES 字串

OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H

InChI 密鑰

OSBLTNPMIGYQGY-UHFFFAOYSA-N

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相关类别

一般說明

TBE（Tris/硼酸/EDTA)缓冲液通常用于核酸电泳。该溶液在弱碱性条件下有效，能够保持DNA去质子化，水溶性，免于降解。该浓缩溶液能够方便地在使用前稀释到1x或0.5x（分子生物级别水）。

應用

稀释到工作浓度后，可用于凝胶电泳。
Tris-硼酸-EDTA缓冲液用于脉冲电场凝胶电泳。
TBE电泳液是DNA和RNA聚丙烯酰胺凝胶电泳最常用的缓冲液。TBE用于非变性或变性（7M 尿素）凝胶。 它通常也用于DNA自测序凝胶。 TBE可以用于琼脂糖凝胶，但是不推荐用于回收核酸的制备胶。
将TBE储存浓缩溶液稀释到1×TBE电泳缓冲液，生成的缓冲液含有89mM Tris-硼酸和2mM EDTA，pH8.3。 5X或10X储存液也可以加入丙烯酰胺/甲叉双丙烯酰胺储存液，用来制成PAGE胶。 为了达到最大的分辨率，推荐电压小于5V/cm(电极单元间距离）。

包裝

4L、10L 和 20L 容量用带龙头分液器包装。

準備報告

用 18 兆欧水制备
采用经生物技术性能认证的 Trizma 碱（产品代码 T6066）、分子生物学试剂硼酸（产品代码 B6768）和 EDTA 二钠盐（产品代码 E5134）制备。

其他說明

TBE 缓冲剂会逐渐沉淀。通常而言，沉淀不会影响性能。

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H360FD

危險分類

Repr. 1B

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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L Orbán et al.
Electrophoresis, 12(4), 233-240 (1991-04-01)
DNA fragments up to 9 kb in size were stacked and separated by polyacrylamide gel electrophoresis, and those up to 50 kb in size by agarose gel electrophoresis, using a discontinuous buffer system. Polyacrylamide gels at pH 8.9, 2 degrees
M Wu et al.
BioTechniques, 24(4), 676-678 (1998-06-13)
A new agarose-based protein electrophoresis gel system is described. The system consists of a highly resolving agarose, MetaPhor XR (FMC BioProducts, Rockland, ME, USA) dissolved in urea and TBE buffer and a stacking gel composed of a high gel-strength agarose
Characterization of a Clavibacter michiganensis subsp. michiganensis population in Israel.
Kleitman, F., Barash, I., Burger, A., Iraki, N.,
European Journal of Plant Pathology, 121, 463-475 (2008)
Josep Balart et al.
Radiation oncology (London, England), 6, 6-6 (2011-01-18)
Radiation-induced DNA double-strand break (DSB) repair can be tested by using pulsed-field gel electrophoresis (PFGE) in agarose-encapsulated cells. However, previous studies have reported that this assay is impaired by the spontaneous DNA breakage in this medium. We investigated the mechanisms
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Nanofluidics represents a promising solution to problems in fields ranging from biomolecular analysis to optical property tuning. Recently a number of simple nanofluidic fabrication techniques have been introduced that exploit the deformability of elastomeric materials like polydimethylsiloxane (PDMS). These techniques
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实验方案

TAE and TBE Running Buffers Recipe & Video

TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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