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HPLC Analysis of Caffeine and Taurine on Ascentis® Express HILIC Column using Evaporative Light Scattering Detection (ELSD, Fast Analysis)

HPLC Analysis of Caffeine and Taurine on Ascentis® Express HILIC Column using Evaporative Light Scattering Detection (ELSD, Fast Analysis) application for HPLC

Materials

Analytical column

Product No.
Description
Pricing

53967-U

Ascentis® Express HILIC, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 5 cm × 3 mm

Standard

Product No.
Description
Pricing

T0625

Taurine

≥99%

56396

Caffeine

certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

CONDITIONS

column

Ascentis Express HILIC, 5 cm x 3.0 mm I.D., 2.7 μm particles (53967-U)

mobile phase

[A] 100 mM ammoniumformate, pH 3.0 with formic acid; [B] acetonitrile; (15:85, A:B)

flow rate

0.6 mL/min

column temp.

25 °C

detector

ELSD, 55 °C, 3.5 bar nitrogen

injection

2 μL

sample

1000 μg/mL in 10:90, water: acetonitrile

Description

Analysis Note

This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of caffeine and taurine. Elution is by aqueous normal phase (ANP) as an increase in organic will increase retention.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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