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Key Documents

T5912

Sigma-Aldrich

Tris Buffered Saline

10X solution

Synonym(s):

TBS

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About This Item

MDL number:
MFCD00132476
UNSPSC Code:
12161700
NACRES:
NA.25

product name

Tris Buffered Saline, 10 ×, solution

sterility

0.2 μm filtered

form

solution

concentration

10 ×

storage temp.

2-8°C

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Application

Tris Buffered Saline has been used as washing buffer for electrodes. It was used to wash microtiter plates for western blot experiments and screening methods.

Other Notes

Washing buffer for alkaline phosphatase or peroxidase conjugates in Western blotting. Working solution will contain 20 mM Tris, pH approx. 7.4, and 0.9% NaCl

Suitability

Suitable for use as a washing buffer for alkaline phosphatase or peroxidase conjugates in dot blot assay.

Related product

Product No.
Description
Pricing

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Rapid prototyping of multifunctional microfluidic cartridges for electrochemical biosensing platforms
Kin J
Sensors and Actuators B, Chemical, 202, 60-66 (2014)
Larry H Stanker et al.
Journal of immunological methods, 336(1), 1-8 (2008-05-03)
Botulinum neurotoxins (BoNT), produced by the anaerobic bacterium Clostridium botulinum, cause severe neuroparalytic disease and are considered the most toxic biological agents known. While botulism is rare in the U.S. it often is fatal if not treated quickly, and recovery
Jingtao Zhong et al.
International journal of molecular medicine, 41(5), 2893-2900 (2018-02-14)
Secretory clusterin (sCLU) is overexpressed in cancer and is associated with resistance to chemotherapy in several types of cancer, including hepatocellular carcinoma (HCC). Sorafenib (SOR), a multikinase inhibitor of Raf/mitogen‑activated protein kinase kinase/extracellular signal‑regulated kinase (ERK) signaling and the receptor
Kim A Tappe et al.
European journal of immunology, 48(12), 2042-2054 (2018-09-28)
"Immunogenic cell death" (ICD) is associated with the emission of so-called damage-associated molecular patterns (DAMPs) which trigger the immune response against dead-cell associated antigens. The secretion of the DAMP, adenosine triphosphate (ATP) has been shown to be autophagy-dependent. Here, we
Larry H Stanker et al.
Methods in molecular biology (Clifton, N.J.), 1318, 69-78 (2015-07-15)
The sandwich immunoassay (sELISA) is an invaluable technique for concentrating, detecting, and quantifying target antigens. The two critical components required are a capture antibody and a detection antibody, each binding a different epitope on the target antigen. The specific antibodies
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