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T2145

Sigma-Aldrich

Tyrode′s Salts

Powder, without sodium bicarbonate, suitable for cell culture

Synonym(s):

Tyrode′s Buffer, Tyrode′s Solution, Tyrode′s buffer

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

product name

Tyrode′s Salts, Without sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

Related Categories

General description

Tyrode′s Salts play an important role in tissue culture. A salt solution′s basic function is to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.

Application

Tyrode′s Salts has been used:
  • for seeding cardiomyocytes culture for optical based phenotyping and imaging studies
  • to incubate 3T3-L1 pre-adipocytes in calcium intracellular assays
  • as a medium for the isolation and culturing of myofiber extensor digitorum longus (EDL) muscle

Quantity

Formulated to contain 9.6 grams of powder per liter of medium.

Reconstitution

Supplement with 1.0 g/L sodium bicarbonate.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Felipe Mora-Bermúdez et al.
eLife, 3 (2014-07-06)
Mitotic spindle orientation is crucial for symmetric vs asymmetric cell division and depends on astral microtubules. Here, we show that distinct subpopulations of astral microtubules exist, which have differential functions in regulating spindle orientation and division symmetry. Specifically, in polarized
Theodora Pavlidou et al.
Stem cells international, 2019, 5980465-5980465 (2019-06-30)
The regeneration of the muscle tissue relies on the capacity of the satellite stem cell (SC) population to exit quiescence, divide asymmetrically, proliferate, and differentiate. In age-related muscle atrophy (sarcopenia) and several dystrophies, regeneration cannot compensate for the loss of
Tomasz Maciej Stepniewski et al.
Chemical science, 12(33), 10990-11003 (2021-09-16)
Brain functions rely on neurotransmitters that mediate communication between billions of neurons. Disruption of this communication can result in a plethora of psychiatric and neurological disorders. In this work, we combine molecular dynamics simulations, live-cell biosensor and electrophysiological assays to
Fernando M Nunez et al.
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Brainstem gliomas are aggressive tumors that are more prevalent in pediatric patients. The location of these tumors makes them inoperable, and currently there is no effective treatment. Recent genomic data revealed the unique biology of these tumors. The following protocol
Nina Kudryashova et al.
PLoS computational biology, 15(3), e1006597-e1006597 (2019-03-19)
Cardiac fibrosis occurs in many forms of heart disease and is considered to be one of the main arrhythmogenic factors. Regions with a high density of fibroblasts are likely to cause blocks of wave propagation that give rise to dangerous
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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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