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SAE0098

Sigma-Aldrich

Lyticase from Arthrobacter luteus

free of DNA contaminants, suitable for Microbiome research, ≥2000 units/mg protein, lyophilized powder

Synonym(s):

(1,3)-β-D-Glucan endohydrolase, 1,3-β-Glucan glucohydrolase

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About This Item

CAS Number:
37340-57-1
MDL number:
MFCD00131560
UNSPSC Code:
12352202
NACRES:
NA.54

biological source

bacterial (Arthrobacter luteus)

form

lyophilized powder

specific activity

≥2000 units/mg protein

feature

DNA free

technique(s)

cell based assay: suitable

suitability

suitable for cell lysis

application(s)

diagnostic assay manufacturing

shipped in

dry ice

storage temp.

−20°C

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General description

Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase, chitinase, zymolase, and gluculase to induce partial spheroplast formation; spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation.

Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species.

Purified Lyticase SAE0098 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.

Biochem/physiol Actions

Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.

Unit Definition

One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

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