L4025
Lyticase from Arthrobacter luteus
lyophilized powder, ≥200 units/mg solid
Synonym(s):
(1,3)-β-D-Glucan endohydrolase, 1,3-β-Glucan glucohydrolase, Bacterial lyticase, Lysing enzyme
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About This Item
Recommended Products
biological source
bacterial (Arthrobacter luteus)
Quality Level
form
lyophilized powder
specific activity
≥200 units/mg solid
technique(s)
cell based assay: suitable
suitability
suitable for cell lysis
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
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Application
Lyticase from Arthrobacter luteus has been used for resuspending cells to extract high-molecular weight DNA for polymerase chain reaction (PCR) fingerprint analyses.
Lyticase from Arthrobacter luteus has been used:
- as a component in spheroplasting buffer to prepare yeast nuclear extracts
- for digestion in sample preparation for β-glucan enzymatic assay using yeast
- to lyse cells for RNA isolation
Biochem/physiol Actions
Lyticase enzyme is frequently used in fungal research, particularly for species identification using polymerase chain reaction (PCR)-based techniques. It can break down β (1→3) and β (1→4) bonds between glucose units.
Lyticase is a lysing enzyme that is used to extract DNA from yeast cells by inducing partial spheroplast formation. Spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast, which are difficult to disrupt because the cell walls may form capsules or resistant spores. Lyticase contains β-(1→;3)-glucan laminaripentaohydrolase along with β-(1→3)-glucanase, protease, and mannanase activities. It is useful with yeast cells such as e.g. Candida, Debaryomyces, Saccharomyces, Saccharomycopsis, Saccharomycodes, Eremothecium, and Schwanniomyces species.
Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.
Unit Definition
One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
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Protocols
This procedure may be used for the determination of Lyticase activity using Baker’s yeast as the substrate.
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