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SAE0092

Sigma-Aldrich

Mutanolysin from Streptomyces globisporus ATCC 21553

free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥4000 units/mg protein (biuret)

Synonym(s):

Mutanolysin

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About This Item

CAS Number:
55466-22-3
MDL number:
MFCD00131635
UNSPSC Code:
12352202
NACRES:
NA.54

biological source

Streptomyces sp. (Streptomyces globisporus ATCC 21553)

form

lyophilized powder

specific activity

≥4000 units/mg protein (biuret)

mol wt

23 kDa
 ~18.5 kDa

feature

DNA free

concentration

≥40% protein

technique(s)

nucleic acid detection: suitable

suitability

suitable for cell lysis

application(s)

life science and biopharma

shipped in

wet ice

storage temp.

−20°C

Application

Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.
Purified Mutanolysin SAE0092 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Mutanolysin is a muralytic enzyme, suitable for usage in isolation of nucleic acids, from Gram-positive bacteria (e.g. Listeria, Lactobacillus, Lactococcus),(8) and also from other bacteria that are difficult to lyse with lysozyme only.

Biochem/physiol Actions

Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus.

Features and Benefits

Suitable for difficult-to-lyse samples.

Microbial DNA free, minimizing DNA contamination from reagents in workflow.

Suitable for the isolation of nucleic acids from Gram-positive bacteria, including Listeria, Lactobacillus, and Lactococcus.

Unit Definition

One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.

Physical form

Lyophilized powder containing Ficoll® and sodium succinate buffer salts

Legal Information

Ficoll is a registered trademark of Cytiva

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Stacey M Broomall et al.
Applied and environmental microbiology, 82(2), 596-607 (2015-11-15)
Effective microbial forensic analysis of materials used in a potential biological attack requires robust methods of morphological and genetic characterization of the attack materials in order to enable the attribution of the materials to potential sources and to exclude other
G B Calandra et al.
Infection and immunity, 28(3), 1033-1037 (1980-06-01)
Group B streptococci, refractory to previously tested muralysins under physiological conditions, were successfully converted to protoplasts by use of a recently describede N-acetyl muramidase, mutanolysin, derived from a streptomycete. Purified enzyme was effective, but crude preparations, although degrading cell walls
K Yokogawa et al.
Antimicrobial agents and chemotherapy, 6(2), 156-165 (1974-08-01)
Mutanolysin partially purified from the culture filtrate of Streptomyces globisporus 1829 consists of two main lytic enzymes with an isoelectric point near pH 8.5 and 10, respectively, and proteolytic enzyme is associated with the latter lytic enzyme. Mutanolysin exhibited maximal
Martin Bastian Pedersen et al.
Journal of bacteriology, 190(14), 4903-4911 (2008-05-20)
Lactococcus lactis is a widely used food bacterium mainly characterized for its fermentation metabolism. However, this species undergoes a metabolic shift to respiration when heme is added to an aerobic medium. Respiration results in markedly improved biomass and survival compared
Caroline Meyer Olesen et al.
Microorganisms, 9(7) (2021-08-08)
Investigation of changes in the skin microbiome following treatment of atopic dermatitis (AD) with dupilumab may provide valuable insights into the skin microbiome as a therapeutic target. The aim of this study is to assess changes in the AD skin
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