P1620

Anti-PRMT1 antibody, Mouse monoclonal

clone PRMT1-171, purified from hybridoma cell culture

• Synonym(s): Anti-Protein-Arginine Methyl Transferase 1
• MDL number: MFCD04975120
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: PRMT1-171, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~42 kDa
• species reactivity: mouse, chicken, bovine, human, canine, hamster, monkey, rat
• concentration: ~2 mg/mL
• technique(s): immunoprecipitation (IP): suitable; indirect ELISA: suitable; western blot: 2 μg/mL using whole cell extract of 293T cells
• isotype: IgG1
• UniProt accession no.: Q99873
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... PRMT1(3276)

Description

General description

Protein arginine methyltransferase 1 (PRMT1) gene is localized on human chromosome 19q13.33. It is part of the type I PRMT family.

Specificity

Does not cross-react with PRMT2, 3, 4, and 5.

Immunogen

full length PRMT1. Does not cross-react with PRMT2, 3, 4, and 5.

Application

Anti-PRMT1 antibody, Mouse monoclonal has been used for Immunofluorescence and Western Blotting.

Biochem/physiol Actions

Protein arginine methyltransferase 1 (PRMT1) is crucial for the formation of asymmetric dimethylarginine. It can methylate Twist-1, a transcription factor. The protein also methylates histones and thus modulates transcription and protein activity. PRMT1 is involved in epithelial-mesenchymal transition and may have a role in cancer.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 C for up to one month. For prolonged storage, freeze in working aliquots at 20 C. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable