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D4309

Sigma-Aldrich

REDTaq® DNA Polymerase

Taq for routine PCR with inert dye, 10X buffer included

Synonym(s):

DNA polymerase with loading dye, Taq DNA polymerase

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About This Item

MDL number:
MFCD01635818
UNSPSC Code:
12352204
NACRES:
NA.55

form

liquid

usage

sufficient for 1000 reactions
 sufficient for 250 reactions
 sufficient for 50 reactions

feature

dNTPs included: no
hotstart: no

concentration

1 unit/μL

technique(s)

PCR: suitable

color

red

input

purified DNA

suitability

suitable for PCR

application(s)

agriculture

shipped in

wet ice

storage temp.

−20°C

General description

REDTaq® DNA Polymerase is a unique blend of Sigma′s quality Taq DNA Polymerase combined with an inert red dye. This dye enables quick visual confirmation of enzyme addition and reaction mixing. An aliquot of the samples (5-10 μl) can then be loaded directly onto an agarose gel for electrophoresis following PCR. The red dye migrates slightly faster than bromophenol blue at about the same rate as a 125 base pair fragment in a 1% agarose gel. Since no additional loading buffers are added to the reaction following PCR, reamplification is possible.
The red dye has no effect on automated or manual sequencing, restriction digestions or other downstream applications. However, if removing the dye is desired, this can easily be accomplished using any standard purification method.

Application

For routine PCR amplifications REDTaq® DNA Polymerase has been used in polymerase chain reaction (PCR) and reverse transcription-polymerase chain reaction (RT-PCR) analysis.

Features and Benefits

  • Same great performance as Taq DNA Polymerase in a more convenient format for high throughput applications.
  • Visual confirmation that not only has the enzyme been added, but that proper mixing has occurred.
  • No additional loading dyes are necessary. An aliquot can be taken directly from the reaction and loaded onto an agarose gel for electrophoresis.

Packaging

Provided with 10X reaction buffer containing MgCl2

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

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