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C5024

Sigma-Aldrich

Carbonic Anhydrase from bovine erythrocytes

non-denaturing PAGE marker

Synonym(s):

Carbonate Dehydratase, Carbonate Hydrolyase

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About This Item

CAS Number:
9001-03-0
Enzyme Commission number:
4.2.1.1 (BRENDA, IUBMB)
EC Number:
232-576-6
MDL number:
MFCD00081467
UNSPSC Code:
12352204
NACRES:
NA.54

Quality Level

200

form

lyophilized powder

mol wt

~29 kDa

suitability

suitable for marker for electrophoresis (non-denaturing PAGE)

storage temp.

−20°C

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Application

This product is used as a marker to determine the molecular mass of an unknown protein in non-denaturing electrophoresis systems. In such systems, proteins often retain their biological activity, thus allowing in-gel detection using specific substrate stains. This technique also facilitates the recovery of the active protein. The product yields 3 bands.

Reconstitution

0.9-1.4 mg/mL after reconstitution with 1 mL of water

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Xiuhong Shan et al.
Journal of computer assisted tomography, 37(1), 22-28 (2013-01-17)
The objective of this study was to investigate the correlation between the degree of necrosis displayed in computed tomography (CT) image and the expression of hypoxic and angiogenesis biomarkers of breast cancer. Forty-four breast cancer cases were examined with CT
Bernardo V Alvarez et al.
BMC cardiovascular disorders, 13, 2-2 (2013-01-10)
Carbonic anhydrase enzymes (CA) catalyze the reversible hydration of carbon dioxide to bicarbonate in mammalian cells. Trans-membrane transport of CA-produced bicarbonate contributes significantly to cellular pH regulation. A body of evidence implicates pH-regulatory processes in the hypertrophic growth pathway characteristic
Laurie C Hofmann et al.
Journal of experimental botany, 64(4), 899-908 (2013-01-15)
The concentration of CO(2) in global surface ocean waters is increasing due to rising atmospheric CO(2) emissions, resulting in lower pH and a lower saturation state of carbonate ions. Such changes in seawater chemistry are expected to impact calcification in
Dina Schütze et al.
Journal of cancer research and clinical oncology, 139(5), 747-754 (2013-01-30)
Carbonic anhydrase IX (CAIX) is involved in pH homeostasis, growth and survival of tumor cells. Besides the membranous form of CAIX, a soluble form is detectable in serum (s-CAIX). Overexpression of CAIX in tumors offers the opportunity for therapeutic strategies
Frédéric D Birkhäuser et al.
Journal of immunotherapy (Hagerstown, Md. : 1997), 36(2), 102-111 (2013-02-05)
The dendritic cell vaccine DC-Ad-GM·CAIX is an active, specific immunotherapy with the potential of providing a safe and effective therapy against renal cell carcinoma (RCC). Using immunocompetent Balb/c mouse models we tested the efficacy and mechanism of the vaccine to
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