MilliporeSigma
All Photos(2)

C6624

Sigma-Aldrich

Carbonic Anhydrase II human

recombinant, expressed in E. coli, buffered aqueous solution

Synonym(s):
CA-II, CA2, Carbonic Anhydrase 2
Enzyme Commission number:
MDL number:
NACRES:
NA.54

recombinant

expressed in E. coli

Quality Level

form

buffered aqueous solution

specific activity

≥5,000 units/mg protein

mol wt

large subunit 17-22 kDa
small subunit 10-12 kDa

concentration

800-1000 μg/mL

NCBI accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CA2(760)

Application

Human carbonic anhydrase II has been used in a study to assess quantitative imaging of mitochondrial and cytosolic free zinc levels in an in vitro model of ischemia/reperfusion. Human carbonic anhydrase II has also been used in a study to investigate a new scrubber concept for catalytic CO2 hydration.

Biochem/physiol Actions

Carbonic anhydrase is a zinc metalloenzyme that catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in vital physiological and pathological processes such as pH and CO2 homeostasis, transport of bicarbonate and CO2, biosynthetic reactions, bone resorption, calcification, and tumorigenicity. It is required for renal acidification. Its absence can lead to osteoporosis, renal tubular acidosis, and cerebral calcification. Therefore, this enzyme is an important target for inhibitors with clinical applications in glaucoma, epilepsy and Parkinson′s disease. In addition, it is being explored as a potential target for obesity and cancer. CAII has a molecular mass of approximately 30 kDa and is primarily present in type II pneumocytes. Due to this unique location it has been speculated that CAII is involved in pulmonary functions such as regulation of fluid secretion and facilitation of CO2 elimation. Sulfonamides, sulfamates and sulfamides are potent inhibitors of CA.

Unit Definition

One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0 °C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)

Physical form

Supplied as a solution in 20 mM Tris, pH 7.5, with 150 mM NaCl.

Storage Class Code

12 - Non Combustible Liquids

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

More Documents

SDS

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service