RDD029

Guanidine thiocyanate

for molecular biology, free-flowing, Redi-Dri^™, ≥99.0%

• Synonym(s): GTC, Guanidine isothiocyanate, Guanidinium rhodanide, Guanidinium thiocyanate
• Linear Formula: NH₂C(=NH)NH₂ · HSCN
• CAS Number: 593-84-0
• Molecular Weight: 118.16
• Beilstein/REAXYS Number: 3563461
• MDL number: MFCD00013027
• UNSPSC Code: 12352107

Properties

• product line: Redi-Dri^™
• Quality Level: 100
• assay: ≥99.0%
• quality: for molecular biology; free-flowing
• mp: 120-122 °C (lit.)
• foreign activity: DNAse, none detected; RNAse, none detected
• SMILES string: SC#N.NC(N)=N
• InChI: 1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H
• InChI key: ZJYYHGLJYGJLLN-UHFFFAOYSA-N

Description

General description

Guanadine thiocyanate (GTC) is a chaotropic agent and strong denaturant that is used in research for protein degredation in vitro. This is useful for DNA or RNA isolation and purification. GTC is a key reagent for inactivation of endogenous DNases and RNase for molecular biology techniques used to isolate nucliec acids from tissues, cells, or viral genomes.

Application

Guanidine thiocyanate is commonly used for research:

• to denature proteins in cell lysis buffer
• to isolate and purify RNA from cells and tissues
• to extract DNA from cellular and viral genomes

Chaotropic agent and strong denaturant; solubilizes cells.

Biochem/physiol Actions

Guanadine thiocyanate is strong protein denaturant commonly combinded with phenol to lyse tissues, cells, and proteins during RNA isolation assay.

Features and Benefits

• Redi-Dri^™ makes working with hygroscopic chemicals easier
• Controls moisture and powder remains free-flowing
• Safer to handle and weigh out for routine use in lab
• Eliminates clumps and maintains product moisture and quality attributes

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• pictograms: GHS05,GHS07
• signalword: Danger
• hcodes: H302 + H312 + H332 - H314 - H412
• pcodes: P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338 + P310
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
• supp_hazards: EUH032
• Storage Class: 8B - Non-combustible, corrosive hazardous materials
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable