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77515

Sigma-Aldrich

Methylene Blue solution

for microscopy

Synonym(s):

Ehrlich’s reagent III, Loeffler’s Methylene Blue

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About This Item

Empirical Formula (Hill Notation):
C16H18ClN3S
CAS Number:
61-73-4
Molecular Weight:
319.85
Beilstein/REAXYS Number:
3641570
MDL number:
MFCD00012111
UNSPSC Code:
12171500
PubChem Substance ID:
329767738
NACRES:
NA.47

grade

for microscopy

form

solution

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | staining: suitable

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria

suitability

Corynebacterium spp.
bacteria
mycobacteria
spore forming microorganisms

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1

InChI key

CXKWCBBOMKCUKX-UHFFFAOYSA-M

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Application

Loefflers Methylene Blue stain is used for the identification of diphteria bacilli, since it differentiates the deeply staining metachromatic granules from the pale blue-staining cytoplasm.

Components

Composition:
methylene blue 0.3 g
ethanol 30 ml
water 100 ml

Other Notes

This product has been replaced by 03978-SIGMA | Methylene Blue solution for microscopy, concentrate according to Ehrlich, concentrated, aqueous solution

Replaced by

Product No.
Description
Pricing

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk_germany

WGK 2

flash_point_f

113.0 °F

flash_point_c

45 °C

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
BCCH2363
BCCG2907
BCCF6236
BCCD4887
BCCC0046

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Jonel Steffen et al.
The British journal of ophthalmology, 98(1), 13-15 (2013-10-26)
To evaluate the diagnostic accuracy of methylene blue used as a non-invasive in vivo stain to detect ocular surface squamous neoplasia (OSSN). A test validation study was performed according to Standards for the reporting of diagnostic accuracy studies (STARD) guidelines
Xiaojuan Bai et al.
Langmuir : the ACS journal of surfaces and colloids, 29(9), 3097-3105 (2013-02-20)
ZnO1-x/graphene hybrid photocatalyst was prepared via a facile in-situ reduction of graphene oxide and ZnO1-x surface defect oxide. The hybrid photocatlayst showed enhanced photocatalytic activity for the photodegradation of methylene blue. The photocorrosion of ZnO1-x was successfully inhibited by graphene
Nicolaas Lucius Gerlach et al.
Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons, 72(4), 666-671 (2014-02-01)
To establish the accuracy of cone-beam computed tomographic (CBCT) views in determining the position and diameter of the mandibular canal. Two freshly frozen cadaver heads, 1 dentate and 1 edentate, were used to acquire CBCT scans. Measurements on cross-sectional CBCT
Q R J G Tummers et al.
European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology, 40(7), 850-858 (2014-05-28)
Despite recent developments in preoperative breast cancer imaging, intraoperative localization of tumor tissue can be challenging, resulting in tumor-positive resection margins during breast conserving surgery. Based on certain physicochemical similarities between Technetium((99m)Tc)-sestamibi (MIBI), an SPECT radiodiagnostic with a sensitivity of
Bram Vivijs et al.
Applied and environmental microbiology, 80(19), 6054-6061 (2014-07-27)
Some members of the family Enterobacteriaceae ferment sugars via the mixed-acid fermentation pathway. This yields large amounts of acids, causing strong and sometimes even lethal acidification of the environment. Other family members employ the 2,3-butanediol fermentation pathway, which generates comparatively
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