Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

More Documents

03978

Sigma-Aldrich

Methylene Blue solution

for microscopy, concentrate according to Ehrlich, concentrated, aqueous solution

Synonym(s):

Methylene blue stain, Ehrlich’s reagent III, Loeffler’s Methylene Blue

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C16H18ClN3S
CAS Number:
61-73-4
Molecular Weight:
319.85
Beilstein/REAXYS Number:
3641570
MDL number:
MFCD00012111
UNSPSC Code:
12171500
PubChem Substance ID:
329747853
NACRES:
NA.47

grade

for microscopy

Quality Level

100

form

liquid

quality

concentrated, aqueous solution

shelf life

~3 yr

concentration

1.5% (1.5 g/ 100 mL)

density

1.0 g/mL at 20 °C

εmax

9.4 at 656-666 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1

InChI key

CXKWCBBOMKCUKX-UHFFFAOYSA-M

Looking for similar products? Visit Product Comparison Guide

General description

Methylene blue is used to stain animal cells to make their nuclei more observable. It is a component of staining media such as Wright′s stain and Jenner′s stain.
Methylene blue, also known as methylthioninium chloride, is a photoactive phenothiazine dye. It is a small molecular weight dye, which is effective against Gram positive and Gram negative bacteria.

Application

Methylene Blue solution has been used to stain human amniotic fluid stem cells to determine cell viability. It has also been used to stain sections to determine the presence of mycobacteria.
Methylene blue is used to stain blood films/smears used in cytology and to stain RNA or DNA for viewing under the microscope or on hybridization membranes.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Antimicrobial Photodynamic Therapy (APDT) Action Based on Nanostructured Photosensitizers
Multifunctional Systems for Combined Delivery Biosensing and Diagnostics (2017)
Real-time fluorescence image-guided oncologic surgery
Advances in Cancer Research (2014)
Interferon-gamma is a crucial activator of early host immune defense against Mycobacterium ulcerans infection in mice
Bieri R, et al.
PLoS Neglected Tropical Diseases, 10(2), e0004450-e0004450 (2016)
Reprogramming human amniotic fluid stem cells to functional pluripotency by manipulation of culture conditions
Moschidou D and Guillot PV
National Protocol Exchange (2012)
Pathogen reduction technology treatment of platelets, plasma and whole blood using riboflavin and UV light
Transfusion Medicine and Hemotherapy (2011)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service