03978

Sigma-Aldrich

Methylene Blue solution

for microscopy, concentrate according to Ehrlich, concentrated, aqueous solution

Synonym(s):
Methylene blue stain, Loeffler’s Methylene Blue, Ehrlich’s reagent III
Empirical Formula (Hill Notation):
C16H18ClN3S
CAS Number:
Molecular Weight:
319.85
Beilstein/REAXYS Number:
3641570
MDL number:
PubChem Substance ID:
NACRES:
NA.47

grade

for microscopy

Quality Level

form

liquid

quality

concentrated, aqueous solution

shelf life

~3 yr

concentration

1.5% (1.5 g/ 100 mL)

application(s)

hematology: suitable
histology: suitable

density

1.0 g/mL at 20 °C

εmax

9.4 at 656-666 nm in water

Featured Industry

Diagnostic Assay Manufacturing

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1

InChI key

CXKWCBBOMKCUKX-UHFFFAOYSA-M

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General description

Methylene blue, also known as methylthioninium chloride, is a photoactive phenothiazine dye. It is a small molecular weight dye, which is effective against Gram positive and Gram negative bacteria.
Methylene blue is used to stain animal cells to make their nuclei more observable. It is a component of staining media such as Wright′s stain and Jenner′s stain.

Application

Methylene blue is used to stain blood films/smears used in cytology and to stain RNA or DNA for viewing under the microscope or on hybridization membranes.
Methylene Blue solution has been used to stain human amniotic fluid stem cells to determine cell viability. It has also been used to stain sections to determine the presence of mycobacteria.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

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