77515

Methylene Blue solution

for microscopy

• Synonym(s): Ehrlich’s reagent III, Loeffler’s Methylene Blue
• Empirical Formula (Hill Notation): C₁₆H₁₈ClN₃S
• CAS Number: 61-73-4
• Molecular Weight: 319.85
• Beilstein/REAXYS Number: 3641570
• MDL number: MFCD00012111
• UNSPSC Code: 12171500
• PubChem Substance ID: 329767738
• NACRES: NA.47

Properties

• grade: for microscopy
• form: solution
• shelf life: limited shelf life, expiry date on the label
• technique(s): microbe id | staining: suitable
• antibiotic activity spectrum: Gram-negative bacteria; Gram-positive bacteria
• suitability: Corynebacterium spp.; bacteria; mycobacteria; spore forming microorganisms
• SMILES string: [Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
• InChI: 1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1
• InChI key: CXKWCBBOMKCUKX-UHFFFAOYSA-M

Description

Application

Loefflers Methylene Blue stain is used for the identification of diphteria bacilli, since it differentiates the deeply staining metachromatic granules from the pale blue-staining cytoplasm.

Components

Composition:
methylene blue 0.3 g
ethanol 30 ml
water 100 ml

Other Notes

This product has been replaced by 03978-SIGMA | Methylene Blue solution for microscopy, concentrate according to Ehrlich, concentrated, aqueous solution

Safety Information

• pictograms: GHS02
• signalword: Warning
• hcodes: H226
• pcodes: P210 - P233 - P240 - P241 - P242 - P243
• Hazard Classifications: Flam. Liq. 3
• Storage Class: 3 - Flammable liquids
• wgk_germany: WGK 2
• flash_point_f: 113.0 °F
• flash_point_c: 45 °C
• ppe: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter