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Key Documents

MAB350

Sigma-Aldrich

Anti-Enkephalin Antibody, clone NOC1

culture supernatant, clone NOC1, Chemicon®

Synonym(s):

Anti-CBF, Anti-CBF2, Anti-HSP-CBF, Anti-NOC1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

culture supernatant

antibody product type

primary antibodies

clone

NOC1, monoclonal

species reactivity

guinea pig, rabbit, rat, hamster

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... CEBPZ(10153)

Specificity

Met5- and Leu5-enkephalin. Displays about 40% cross-reactivity with C-terminal extended Met-enkephalin hexapeptides and 7% cross-reactivity with the extended heptapeptide (-Arg-Phe-OH) but does not recognize pro-enkephalin or other endogenous peptides (Cuello, 1984). By immunohistochemistry the antibody lables all sites that are known to exppress enkephalin and shows no cross-reactivity to beta-endorphin or dynorphin (Cuello, 1984; MacMillan and Cuello, 1986).

Immunogen

Leu5-enkephalin conjugated to BSA.

Application

Detect Enkephalin using this Anti-Enkephalin Antibody, clone NOC1 validated for use in IC, IH.
Immunohistochemistry: 1:100-1:400 on 10 μm cryostat sections of rat and guinea-pig brain stem using fluorescently labled secondary antibodies for detection (Cuello, 1984; MacMillan and Cuello, 1986).

Immunocytochemistry: 1:300 on primary cultures of rat striatal and hippocampal neurons using a Cy2 or Cy3 conjugated secondary antibody for detection (Saudou, 1998).

Radioimmunoassay (Cuello, 1984)

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neuroinflammation & Pain

Hormones & Receptors

Physical form

Lyophilized Supernatant, preservative free.

Storage and Stability

Lyophilized antibody can be kept at 2-8°C for up to 3 months and should be kept at -20°C for long term storage. Reconstitute with 100μL sterile DI water. Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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F Saudou et al.
Cell, 95(1), 55-66 (1998-10-20)
The mechanisms by which mutant huntingtin induces neurodegeneration were investigated using a cellular model that recapitulates features of neurodegeneration seen in Huntington's disease. When transfected into cultured striatal neurons, mutant huntingtin induces neurodegeneration by an apoptotic mechanism. Antiapoptotic compounds or
Electroacupuncture modulation of reflex hypertension in rats: role of cholecystokinin octapeptide.
Li, M; Tjen-A-Looi, SC; Guo, ZL; Longhurst, JC
American Journal of Physiology. Regulatory, Integrative and Comparative Physiology null
Identification of urocortin 3 afferent projection to the ventromedial nucleus of the hypothalamus in rat brain.
Chen, P; Lin, D; Giesler, J; Li, C
The Journal of Comparative Neurology null
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