All Photos(3)

G1890

Sigma-Aldrich

Gelatin from porcine skin

powder, gel strength ~300 g Bloom, Type A, BioReagent, suitable for electrophoresis, suitable for cell culture

CAS Number:
EC Number:
MDL number:
NACRES:
NA.75

Quality Level

biological source

Porcine skin

sterility

sterile

type

Type A

product line

BioReagent

form

powder

packaging

pkg of 1 kg
pkg of 100 g
pkg of 500 g

application(s)

cell culture | mammalian: suitable
electrophoresis: suitable

surface coverage

100‑200 μg/cm2

solubility

H2O: soluble 50 mg/mL, clear to hazy, faintly yellow

shipped in

ambient

storage temp.

room temp

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Gelatin from porcine skin is generated from the acidic digestion of collagen and is referred as type A. It comprises majorly glycine, proline and hydroxyproline. Gelatin takes up random coil structure after digestion from the triple helical collagen. It differs from type B bovine gelatin at the N-terminal sequence.

Application

Gelatin has been used in many applications. It has been used in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has been used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, gelatin can be used as a suspending and encapsulating agent, among other applications. This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Packaging

1 kg in poly bottle
100, 500 g in poly bottle

Components

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.

Caution

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Preparation Note

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gel strength of approximately 300.

Storage Class Code

11 - Combustible Solids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Thermosensitive chitosan-gelatin-glycerol phosphate hydrogel as a controlled release system of ferulic acid for nucleus pulposus regeneration
Cheng YH, et al.
Biomaterials, 32(29), 6953-6961 (2011)
Małgorzata Kozikowska et al.
Scientific reports, 9(1), 11969-11969 (2019-08-21)
The retention of the embryo in the transfer catheter after embryo transfer (ET) during in vitro fertilization is a very common phenomenon, encountered by even the most experienced operators, and embryos retained in the transfer catheter or its sleeve require...
In vitro derivation of chondrogenic cells from human embryonic stem cells
Human Embryonic Stem Cell Protocols, 77(1), 317-331 (2009)
Rodolphe Soret et al.
The Journal of clinical investigation, 125(12), 4483-4496 (2015-11-17)
Hirschsprung's disease (HSCR) is a severe congenital anomaly of the enteric nervous system (ENS) characterized by functional intestinal obstruction due to a lack of intrinsic innervation in the distal bowel. Distal innervation deficiency results from incomplete colonization of the bowel...
The effects of GDF-5 and uniaxial strain on mesenchymal stem cells in 3-D culture
Farng E, et al.
Clinical Orthopaedics and Related Research, 466(8), 1930-1937 (2008)

Articles

Photo-Crosslinkable Gelatin Hydrogel: Versatile Materials for (High Resolution) Additive Manufacturing

Discussion of synthetic modifications to gelatin, improving the three-dimensional (3D) print resolution, and resulting material properties.

Attachment Factors for 3-Dimensional Cell Culture

Attachment Factors for 3-Dimensional Cell Culture

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues.

Three-Dimensional Printing of Tissue Engineering Scaffolds

We will explore the technological advances that have contributed toward the progress of 3DP of tissue engineering scaffolds, current materials used to create 3DP scaffolds, and the challenges that remain.

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service