ES-006

EmbryoMax^® 0.1% Gelatin Solution

The EmbryoMax 0.1% Gelatin Solution is available in a 500 mL format and may be used for routine mouse embryonic stem cell culture applications.

• Synonym(s): Porcine Gelatin Coating Solution, porcine gelatin
• UNSPSC Code: 12352207
• eCl@ss: 32160801
• NACRES: NA.75

Properties

• Quality Level: 100; 200
• form: liquid
• manufacturer/tradename: Specialty Media; EmbryoMax^®
• technique(s): cell culture | stem cell: suitable
• input: sample type induced pluripotent stem cell(s); sample type: mouse embryonic stem cell(s)

Description

Application

Procedure for Coating Multiwell Dishes

1. Add the appropriate amounts of 0.1% Gelatin in Sterile Water to each well of the plate as indicated in the table below:

Wells	_	Amount/Well
96		100 μL
48		300 μL
24		0.5 mL
12		1.0 mL
6		1.5-2.0 mL

2. Leave the gelatin solution on the wells for at least 30 minutes at room temperature, with dish lids on.

3. Remove the gelatin solution and discard.

4. Immediately add cells to the dish.

Note: Do not allow the dishes to dry before adding cells.

Physical form

EmbryoMax 0.1% Gelatin Solution. Gelatin is sourced from porcine. Product is ready to use for plating.

Storage and Stability

Store at ambient temperature for upto 2 years

Legal Information

EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: nwg
• flash_point_c: Not applicable