LC/MS Analysis of Clenbuterol In Plasma on Astec^® CHIROBIOTIC^® T with Phospholipid Removal using HybridSPE^® Phospholipid

sample preparation

SPE (Solid Phase Extraction)

sample/matrix

rat plasma spiked with clenbuterol enantiomers at 10 ng/mL

SPE well plate

HybridSPE-Precipitation 96-well Plate, 50 mg/well (575656-U)

sample addition

100 μL spiked rat plasma followed by 300 μL 1% formic acid in acetonitrile. Mix by vortexing the HybridSPE-PPT plate briefly.

elution

apply vacuum

column

CHIROBIOTIC T, 10 cm x 2.1 mm I.D., 5 μm particles (12018AST)

mobile phase

10 mM ammonium formate in methanol

flow rate

0.3 mL/min

column temp.

30 °C

detector

ABI 3200 QT; ESI(+), MRM: 184/104 m/z (phospholipids); 277.2/203.1 m/z (clenbuterol)

injection

10 μL