Analiza HPLC nukleozydów w 2445 Express OH5 (tryb HILIC)

column

Ascentis Express OH5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53757-U)

mobile phase

[A] 5 mM ammonium acetate, pH 5.0 with acetic acid in 95:5, acetonitrile:water; [B] 5 mM ammonium acetate, pH 5.0 with acetic acid in 80:20, acetonitrile:water

gradient

0% B held for 1 min; to 100% B in 10 min; held at 100% B for 1 min

flow rate

0.3 mL/min

column temp.

25 °C

detector

UV, 250 nm

injection

2 μL

sample

10 - 100 μg/mL in 95:5, acetonitrile:water (pH of buffer stock (in water) was adjusted before further dilution with water and/or acentonitrile)