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HPLC Analysis of Morphine and Glucuronide Metabolites on Discovery® HS F5

HPLC Analysis of Morphine and Glucuronide Metabolites on Discovery® HS F5 application for HPLC

Materiały

kolumna analityczna

Numer produktu
Opis
Cennik

Discovery® HS F5 HPLC Column

5 μm particle size, L × I.D. 5 cm × 4.6 mm

standardowe

Numer produktu
Opis
Cennik

Morphine-3-β-D-glucuronide solution

100 μg/mL in methanol: water ( 50:50(v/v)), ampule of 1 mL, certified reference material, Cerilliant®

Morphine solution

100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Morphine-6-β-D-glucuronide solution

100 μg/mL in methanol: water (1:1), ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Discovery HS F5, 5 cm x 4.6 mm I.D., 5 μm particles (567513-U)

mobile phase

[A] 10 mM ammonium acetate, pH 6.8 (unadjusted); [B] acetonitrile

gradient

0% B for 1 min, to 30% B in 4 min, held 3 min

flow rate

1 mL/min

column temp.

40 °C

detector

UV, 280 nm

injection

10 μL

sample

50 μg/mL in final mobile phase

Opis

Informacje prawne

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany