567513-U

Discovery^® HS F5 (5 µm) HPLC Columns

L × I.D. 5 cm × 4.6 mm, HPLC Column

• Synonim(y): Discovery Pentafluorophenyl PFP HPLC Column
• Kod UNSPSC: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Właściwości

• product name: Discovery^® HS F5 HPLC Column, 5 μm particle size, L × I.D. 5 cm × 4.6 mm
• Materiały: stainless steel column
• agency: suitable for USP L43
• linia produktu: Discovery^®
• Właściwości: endcapped
• producent / nazwa handlowa: Discovery^®
• opakowanie: 1 ea of
• zakres etykietowania: 12% Carbon loading
• Parametry: ≤70 °C temp. range; 400 bar pressure (5801 psi)
• metody: HPLC: suitable; LC/MS: suitable
• dł. × śr. wewn.: 5 cm × 4.6 mm
• powierzchnia: 300 m²/g
• pokrycie powierzchni: 4 μmol/m²
• zanieczyszczenia: <10 ppm metals
• macierz: silica gel, high purity, spherical particle platform; fully porous particle
• grupa aktywna macierzy: PFP (pentafluorophenyl) phase
• wielkość cząstki: 5 μm
• wielkość porów: 120 Å
• operating pH range: 2-8
• Zastosowanie: food and beverages
• metoda separacji: reversed phase

Opis

Opis ogólny

Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log P_o/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.

The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.

Zastosowanie

• Optimization and validation of a bioanalytical HPLC-UV technique for simultaneous determination of underivatized phenylalanine and tyrosine in the blood for phenylketonuria diagnosis and monitoring.: This study utilizes the Discovery^® HS F5 HPLC Column to develop an HPLC-UV method for the simultaneous determination of phenylalanine and tyrosine in blood, significantly advancing the diagnostic and monitoring capabilities for phenylketonuria (Rabti et al., 2024).


• A new RP-HPLC method as an auxiliary tool for optimization of sample preparation procedures for tracing of PPCPs of different hydrophilicities.: Demonstrates the application of the Discovery^® HS F5 HPLC Column in optimizing sample preparation techniques for the detection of pharmaceuticals and personal care products in environmental samples, enhancing the analysis of diverse hydrophilic compounds (Portillo-Castillo et al., 2021).


• "Heart-cut" bidimensional achiral-chiral liquid chromatography applied to the evaluation of stereoselective metabolism, in vivo biological activity and brain response to chiral drug candidates targeting the central nervous system.: Utilizes the Discovery^® HS F5 HPLC Column in a novel bidimensional chromatographic setup to study the stereoselective metabolism of CNS-targeting chiral drugs, contributing to a deeper understanding of their pharmacological profiles (Battisti et al., 2016).


• HPLC method development, validation, and impurity characterization of a potent antitumor indenoisoquinoline, LMP776 (NSC 725776).: This research employs the Discovery^® HS F5 HPLC Column for developing an HPLC method to identify and characterize impurities in the antitumor drug LMP776, ensuring drug purity and compliance with regulatory standards (Wang et al., 2016).


• Development studies on determination of preservatives decomposition products.: Showcases the application of the Discovery^® HS F5 HPLC Column in the analysis of decomposition products of preservatives, enhancing the safety assessments of food and pharmaceutical products (Zabrzewska et al., 2014).

Cechy i korzyści

• Unique selectivity
• Similar retention to C18 (sometimes requires stronger mobile phase)
• Excellent peak shape
• Stable, low-bleed LC-MS separations
• Scalable separations from 3 to 10μm particle sizes

Polecane produkty

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Informacje prawne

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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