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HPLC Analysis of Carotenoids by Normal Phase Chromatography on Ascentis® Si

HPLC Analysis of Carotenoids by Normal Phase Chromatography on Ascentis® Si application for HPLC

Materiały

Standardowe

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Cennik

Xanthophyll

from marigold

Astaxanthin

≥97% (HPLC), from Blakeslea trispora

kolumna analityczna

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Cennik

Ascentis® Si HPLC Column

5 μm particle size, L × I.D. 15 cm × 4.6 mm

składnik fazy ruchomej

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2-Propanol

99.5%, HPLC grade

CONDITIONS

column

Ascentis Si, 15 cm x 4.6 mm I.D., 5 μm particles (581512-U)

mobile phase

[A] hexane; [B] ethyl acetate; [C] isopropanol; (85:10:5, A:B:C)

flow rate

1.0 mL/min

column temp.

30 °C

detector

UV, 450 nm

injection

10 μL

sample

50-100 μg/mL hexane

Opis

Komentarz do analizy

Ascentis silica is used in the analysis of naturally occurring carotenoid pigments in the form of alcohols, xanthophyll and astaxanthin.

Informacje prawne

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany