HPLC Analysis of Benzodiazepines on Ascentis® Express Phenyl-Hexyl with Acetonitrile Mobile Phase
Materiały
kolumna analityczna
Numer produktu
Opis
Cennik
Ascentis® Express Phenyl-Hexyl, 2.7 μm HPLC Column
2.7 μm particle size, L × I.D. 5 cm × 4.6 mmstandardowe
Numer produktu
Opis
Cennik
Oxazepam solution
1 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Alprazolam solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Clonazepam solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Diazepam solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express Phenyl-Hexyl, 5 cm x 4.6 mm I.D., 2.7 μm particles (53348-U)
mobile phase
[A] water; [B] acetonitrile; (62:38, A:B)
flow rate
1.4 mL/min
pressure
2600 psi (179 bar)
column temp.
35 °C
detector
UV, 250 nm
injection
2 μL
sample
0.1 g/mL of each in 20% methanol
Opis
Komentarz do analizy
The Phenyl-Hexyl phase of the Ascentis Express product line displays the best selectivity of this sample; selectivity is markedly affected by choice of organic modifier. The application also demonstrates feasibility of fast LC with Ascentis Express by virtue of low backpressure and high efficiency.
Informacje prawne
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany