HPLC Analysis of Asparagine Enantiomers on Astec^® CHIROBIOTIC^® T (25 °C)

column

CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

[A] water); [B] methanol; [C] formic acid; (30:70:0.02, A:B:C)

flow rate

1 mL/min

detector

UV, 205 nm

column temp.

25 °C

injection

10 μL

sample

300 μg /mL in 30:70 water:methanol