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Merck

822854

TSKgel® SuperSW mAb HR (4 µm) HPLC Column

L x I.D. 30 m × 7.8 mm, diol phase

Synonim(y):

TSKgel® Size Exclusion (SW-Type) HPLC Column

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About This Item

Kod UNSPSC:
41115711

product name

TSKgel® SuperSW mAb HR HPLC Column, phase diol, L × I.D. 30 m × 7.8 mm, 4 μm particle size

Materiały

stainless steel

agency

suitable for USP L20
suitable for USP L59

linia produktu

TSKgel®

producent / nazwa handlowa

Tosoh SuperSW mAb HR, 22854

Parametry

≤0.5 M salt concentration
0-100% aqueous soluble organic solvents
1 mL/min flow rate
10-30 °C temperature
120 bar max. pressure

metody

HPLC: suitable

dł. × śr. wewn.

30 m × 7.8 mm

macierz

silica particle platform

grupa aktywna macierzy

diol phase

wielkość cząstki

4 μm

wielkość porów

250 Å pore size

pH robocze

2.5-7.5

metoda separacji

size exclusion (SEC)

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Opis ogólny

The TSKgel SuperSW and UltraSW mAb columns consist of three specialized columns designed for the separation and analysis of monoclonal antibodies (mAb). While mAbs can be analyzed using many different modes of HPLC, size exclusion is best to determine the level of aggregation, dimer formation, and fragmentation, making it the best method for heterogeneity studies. TSKgel SuperSW mAb HR columns are optimized for high resolution analysis of mAb monomer and dimer; these 30 cm x 7.8 mm ID slica-based stainless steel columns offer reduced lot-to-lot variation, long column life, reduction of unspecified adsorption, and superior recovery of aggregates. The TSKgel SuperSW mAb columns utilize a unique pore-controlled technology, which produces a shallow calibration curve in the molecular weight region of a typical monoclonal antibody. The calibration curve for the TSKgel SuperSW mAb HR column is similar to that of the industry-leading TSKgel G3000SWxl column and has a shallower slope than the TSKgel UltraSW Aggregate column around the molecular weight range of gamma-globulin. This shallow calibration curve produces high resolution separations.

Zastosowanie

TSKgel® superSW mAb HR HPLC column may be used in the separation of monoclonal antibodies using size exclusion chromatography (SEC).

Postać fizyczna

Shipped in 0.05% NaN3 and 0.1 M Na2SO4 in 0.1 M phosphate buffer, pH 6.7.

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Informacje prawne

TSKgel is a registered trademark of Tosoh Corporation
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Certyfikaty analizy (CoA)

Lot/Batch Number

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Oliver Seifert et al.
mAbs, 11(5), 919-929 (2019-04-06)
Multivalent mono- or bispecific antibodies are of increasing interest for therapeutic applications, such as efficient receptor clustering and activation, or dual targeting approaches. Here, we present a novel platform for the generation of Ig-like molecules, designated diabody-Ig (Db-Ig). The antigen-binding
Alexander Rau et al.
mAbs, 13(1), 1902034-1902034 (2021-03-24)
Dual targeting of surface receptors with bispecific antibodies is attracting increasing interest in cancer therapy. Here, we present a novel bivalent and bispecific antagonistic molecule (Dab-Fc) targeting human epidermal growth factors 2 and 3 (HER2 and HER3) derived from the

Produkty

Size-exclusion chromatography (SEC) columns and ready-to-use standards facilitate method development and increase robustness of protein SEC methods.

Kolumny do chromatografii wykluczania (SEC) i gotowe do użycia wzorce ułatwiają opracowywanie metod i zwiększają niezawodność metod SEC dla białek.

Protokoły

Calibration Curves for TSKgel® SW mAb Columns

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