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ZRB1274

Sigma-Aldrich

Anti-GSDMD Antibody, clone 1D11 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Synonim(y):

Gasdermin-D;Gasdermin domain-containing protein 1

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About This Item

Kod UNSPSC:
12352203
NACRES:
NA.41

pochodzenie biologiczne

rabbit

Poziom jakości

rekombinowane

expressed in HEK 293 cells

białko sprzężone

unconjugated

forma przeciwciała

purified antibody

rodzaj przeciwciała

primary antibodies

klon

1D11, recombinant monoclonal

opis

recombinant, expressed in HEK 293 cells

linia produktu

ZooMAb® learn more

Postać

lyophilized

masa cząsteczkowa

calculated mol wt 52.80 kDa
observed mol wt ~50 kDa

oczyszczone przez

using Protein A

reaktywność gatunkowa

human, mouse

opakowanie

antibody small pack of 25 μL

charakterystyka ekologicznej alternatywy

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

rozszerzona walidacja

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

metody

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

izotyp

IgG

sekwencja epitopowa

Internal

numer dostępu Protein ID

numer dostępu UniProt

kategoria ekologicznej alternatywy

Warunki transportu

ambient

temp. przechowywania

2-8°C

docelowa modyfikacja potranslacyjna

unmodified

Opis ogólny

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specyficzność

Clone 1D11 is a Rabbit recombinant monoclonal antibody that specifically detects Gasdermin-D. It targets an epitope within 25 amino acids from the internal region.

Immunogen

KLH-conjugated linear peptide corresponding to 25 amino acids from the internal region of human Gasdermin-D.

Zastosowanie

Quality Control Testing

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected GSDMD in A431 cell lysate.

Tested applications

Affinity Binding Assay: A representative lot of this antibody bound GSDMD with a KD of 3.0 x 10-7 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected GSDMD in human stomach tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GSDMD in A431, HeLa, HUVEC, and NIH3T3 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Opis wartości docelowych

Gasdermin-D (UniProt: P57764; also known as Gasdermin domain-containing protein 1) is encoded by the GSDMD1 (also known as DFNA5L, GSDMDC1, FKSG10) gene (Gene ID: 79792) in human. Gasdermin-D is a member of the gasdermin family of proteins that promotes inflammatory cell death (pyroptosis) in response to microbial infection and danger signals. Its expression is observed in the suprabasal cells of esophagus, as well as in the isthmus/neck, pit, and gland of the stomach. Gasdermin-2 consists of a 31 kDa N-terminal and a 22 kDa C-terminal domain that are separated by a linker region. Under normal conditions, the full-length Gasdermin-D is inactive as the linker loop between the N-terminal and C-terminal domains stabilizes the overall conformation of the full-length protein and allows the C-terminal domain to fold back on and auto-inhibit the N-terminal Gasdermin-D (Gasdermin-N). Upon interdomain cleavage by the action of caspase-1 and 4 at Asp275, the autoinhibition is relieved and Gasdermin-N cytotoxicity is triggered. Gasdermin-N is reported to form membrane pore that lead to cytokine release and pyroptosis. It also displays bactericidal activity. Gasdermin-N released from pyroptotic cells into the extracellular milieu can bind to and kills both Gram-negative and Gram-positive bacteria, without harming neighboring mammalian cells. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Lieberman, J., et al. (2019). Sci. Immunol. 4(39); eaav1447; Liu, X., et al. (2016). Nature. 535(7610); 153-158).

Postać fizyczna

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Rekonstytucja

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Przechowywanie i stabilność

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informacje prawne

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

13 - Non Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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