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TOX4
In Vitro Toxicology Assay Kit, Neutral Red based
Synonim(y):
NR uptake assay, Neutral red assay
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About This Item
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liquid
zastosowanie
kit sufficient for 1,000 tests
opakowanie
pkg of 1 kit
warunki przechowywania
dry at room temperature
λmaks.
540 nm
Zastosowanie
cell analysis
detection
metoda wykrywania
colorimetric
temp. przechowywania
2-8°C
Opis ogólny
For spectrophotometric measurement of viable cells. Absorbance of converted dye is measured at a wavelength of 540 nm.
Zastosowanie
In Vitro Toxicology Assay Kit, Neutral Red based has been used:
- to measure the lysosomal enzyme activity in HB8902 cells
- to study the cell viability of human alveolar epithelial cells
- to study the cell viability in fibroblasts
Działania biochem./fizjol.
Neutral red (NR) is a red dye that accumulates in the lysosome by actively transferring across the cell membranes. The dye is extracted and the uptake is quantitated by spectroscopy. The NR uptake assay is the most commonly used cytotoxicity test. It is widely applicable in environmental and biomedical studies to estimate the number of viable cells in culture. The NR dye in vitro tissue culture studies is used in assessing viral cytopathogenicity and detection of toxic compounds.
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Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Carc. 1B - Flam. Liq. 3 - Skin Sens. 1
Kod klasy składowania
3 - Flammable liquids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
73.4 °F
Temperatura zapłonu (°C)
23 °C
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In vitro cellular & developmental biology : journal of the Tissue Culture Association, 27A(3 Pt 1), 239-244 (1991-03-01)
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