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MAK066

Sigma-Aldrich

Lactate Dehydrogenase Activity Assay Kit

sufficient for 500 colorimetric tests

Synonim(y):

LDH Activity Assay Kit, LDH Assay, Lactic Dehydrogenase Activity Assay Kit

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About This Item

Kod UNSPSC:
12161503
NACRES:
NA.84

zastosowanie

sufficient for 500 colorimetric tests

metoda wykrywania

colorimetric

powiązane choroby

hematological disorder; cancer

temp. przechowywania

−20°C

informacje o genach

human ... LDHA(3939) , LDHB(3945)
mouse ... LDHA(16828) , LDHB(16832)
rat ... LDHA(24533) , LDHB(24534)

Opis ogólny

The new Lactate Dehydrogenase Assay Kit, MAK464, is now available! Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate. Cells release LDH into the bloodstream after tissue damage or red blood cell hemolysis. Since LDH is a fairly stable enzyme, it has been widely used to evaluate the presence of damage and toxicity of tissue and cells. LDH is also elevated in certain pathological conditions such as cancer. Quantification of LDH has a broad range of applications.




Zastosowanie

Lactate Dehydrogenase Activity Assay Kit has been used for measuring the activity of lactate dehydrogenase (LDH) in the cell culture medium.
Lactate Dehydrogenase Activity Assay Kit has been used to determine the concentration of lactate dehydrogenase in samples.

Przydatność

Suitable for the detection of L(+)-Lactate in biological samples such as serum, plasma, cells, culture and fermentation media.

Zasada

In this kit, LDH reduces NAD to NADH, which is specifically detected by colorimetric (450 nm) assay. The LDH Activity Assay kit quantifies LDH activity in variety of biological samples. The assay is quick, convenient, and sensitive.
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Piktogramy

Corrosion
GHS05

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

H314

Klasyfikacja zagrożeń

Eye Dam. 1 - Skin Corr. 1B

Kod klasy składowania

8A - Combustible, corrosive hazardous materials

Klasa zagrożenia wodnego (WGK)

WGK 3

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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Odwiedź Bibliotekę dokumentów

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Food research international (Ottawa, Ont.), 97, 191-198 (2017-06-05)
Human gut flora-mediated non-digestible fraction of spent coffee grounds (hgf-NDSCG) was evaluated for its chemopreventive effect and molecular mechanisms involved on human colon adenocarcinoma HT-29 cell survival using two different microbiota source [lean (L) and overweight (OW)]. The source of
Adjimon G Lokossou et al.
Biology of reproduction, 102(1), 185-198 (2019-07-19)
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Gustav Holmgren et al.
Toxicology, 328, 102-111 (2014-12-23)
Doxorubicin is a chemotherapeutic agent indicated for the treatment of a variety of cancer types, including leukaemia, lymphomas, and many solid tumours. The use of doxorubicin is, however, associated with severe cardiotoxicity, often resulting in early discontinuation of the treatment.
Anti-Oxidative Effect of Genistein and Mangiferin on Sodium Fluoride Induced Oxidative Insult of Renal Cells: A Comparative Study
Pritam S, et al.
Biomarkers journal., 2(1-10) (2015)
Patuma Sinthujaroen et al.
BBA clinical, 2, 103-111 (2015-01-06)
Billions of cells undergo apoptosis each day in the average normal adult. The ability to readily assess the degree of apoptosis in human diseases is hampered by the lack of sensitive and specific serum biomarkers of apoptosis. Fortilin is a
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Produkty

Aerobic Glycolysis and the Warburg Effect

We presents an article about the Warburg effect, and how it is the enhanced conversion of glucose to lactate observed in tumor cells, even in the presence of normal levels of oxygen. Otto Heinrich Warburg demonstrated in 1924 that cancer cells show an increased dependence on glycolysis to meet their energy needs, regardless of whether they were well-oxygenated or not.

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