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Merck

CGD1

Sigma-Aldrich

Cell Growth Determination Kit, MTT based

Synonim(y):

Mitochondrial activity assay

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About This Item

Kod UNSPSC:
12352207
NACRES:
NA.75

opakowanie

pkg of 1 kit

Poziom jakości

metody

UV/Vis spectroscopy: suitable
protein quantification: suitable

Amax

570 nm

Zastosowanie

cell analysis
detection

metoda wykrywania

colorimetric

Warunki transportu

dry ice

temp. przechowywania

−20°C

Opis ogólny

Cell Growth Determination Kit (MTT based) is designed for the spectrophotometric measurement of cell growth as a function of mitochondrial activity in living cells. The MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) system is a simple, accurate, reproducible means of measuring the activity of living cells via mitochondrial dehydrogenase activity.

Zastosowanie

Cell Growth Determination Kit, MTT based has been used:
  • to measure the cell viability of preosteoblastic cells
  • in MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay of normal human prostatic stromal and epithelial cell lines
  • to determine the cell viability of primary cervical epithelial cells

Opakowanie

Each kit includes 5 vials each containing 1 mL of 5 mg/ml MTT in RPMI (without phenol red) and 50 mL of MTT solvent (0.1 N HCl in anhydrous isopropanol). Volumes of kit components have been optimized for cultures grown in multi-well plates. MTT solutions is added at 10% of total culture volume, thus the number of tests per kit is dependent upon culture vessel and/or volume.
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Hasło ostrzegawcze

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Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Klasyfikacja zagrożeń

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Organy docelowe

Central nervous system

Kod klasy składowania

3 - Flammable liquids

Temperatura zapłonu (°F)

53.6 °F - closed cup

Temperatura zapłonu (°C)

12 °C - closed cup


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Odwiedź Bibliotekę dokumentów

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