M5655

Thiazolyl Blue Tetrazolium Bromide

powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)

• Synonim(y): 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, MTT, Methylthiazolyldiphenyl-tetrazolium bromide
• Wzór empiryczny (zapis Hilla): C₁₈H₁₆BrN₅S
• Numer CAS: 298-93-1
• Masa cząsteczkowa: 414.32
• Beilstein: 4081397
• Numer WE: 206-069-5
• Numer MDL: MFCD00011964
• Kod UNSPSC: 12352207
• Identyfikator substancji w PubChem: 24897030
• NACRES: NA.75

Właściwości

• linia produktu: BioReagent
• Poziom jakości: 200
• Próba: ≥97.5% (HPLC)
• Formularz: powder
• metody: cell culture | insect: suitable; cell culture | mammalian: suitable
• mp: 195 °C (dec.) (lit.)
• rozpuszczalność: H₂O: 5 mg/mL
• temp. przechowywania: 2-8°C
• ciąg SMILES: [Br-].Cc1nc(sc1C)-[n+]2nc(nn2-c3ccccc3)-c4ccccc4
• InChI: 1S/C18H16N5S.BrH/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16;/h3-12H,1-2H3;1H/q+1;/p-1
• Klucz InChI: AZKSAVLVSZKNRD-UHFFFAOYSA-M

Opis

Zastosowanie

Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm.

Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.

Thiazolyl Blue Tetrazolium Bromide has been used as a colorimetric metabolic activity indicator in cell viability assays (MTT assay). It has also been used to determine cell proliferation.

Inne uwagi

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08,GHS07
• Hasło ostrzegawcze: Warning
• Zwroty wskazujące rodzaj zagrożenia: H315,H319,H335,H341
• Zwroty wskazujące środki ostrożności: P202 - P261 - P264 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Klasyfikacja zagrożeń: Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - STOT SE 3
• Organy docelowe: Respiratory system
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)