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Key Documents

STRN50

Sigma-Aldrich

Spectrum Plant Total RNA Kit

greener alternative

sufficient for 50 purifications

Synonim(y):

Plant total RNA extraction kit, Plant total RNA isolation kit, Plant total RNA purification kit

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych


About This Item

Kod UNSPSC:
41105501
NACRES:
NA.52

zastosowanie

sufficient for 50 purifications

Poziom jakości

charakterystyka ekologicznej alternatywy

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

metody

RNA purification: suitable

kategoria ekologicznej alternatywy

temp. przechowywania

15-25°C

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Opis ogólny

The Spectrum Plant Total RNA Kit utilizes a lysis and binding chemistry and a convenient column-based ′bind-wash-and-elute′ format to purify up to 100 μg of total RNA from 100 mg of tissue in about 30 minutes. Typical yields range from 20–60 μg.

After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants.

Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry.

Zastosowanie

Spectrum® Plant Total RNA Kit has been used to isolate RNA from ground fiber tissue of cultured cotton fiber, from neem tissues and from Sarracenia, an insectivorous plant.

Cechy i korzyści

  • Yields up to 60 μg of pure concentrated RNA per prep
  • Efficient protocol allows for RNA purification in 30 min or less
  • Specially designed for research with difficult plant tissues

Informacje prawne

SabreLite is a registered trademark of Pelican Products, Inc.
Spectrum is a trademark of Sigma-Aldrich Co. LLC
This page may contain text that has been machine translated.

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Klasyfikacja zagrożeń

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral

Organy docelowe

Liver,Heart

Kod klasy składowania

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials


Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

S B Gould et al.
Proceedings. Biological sciences, 286(1909), 20191491-20191491 (2019-08-23)
Pyruvate : ferredoxin oxidoreductase (PFO) and iron only hydrogenase ([Fe]-HYD) are common enzymes among eukaryotic microbes that inhabit anaerobic niches. Their function is to maintain redox balance by donating electrons from food oxidation via ferredoxin (Fd) to protons, generating H2
Emir Islamovic et al.
Molecular plant-microbe interactions : MPMI, 28(1), 42-54 (2014-09-17)
Ustilago maydis, causal agent of corn smut disease, is a dimorphic fungus alternating between a saprobic budding haploid and an obligate pathogenic filamentous dikaryon. Maize responds to U. maydis colonization by producing tumorous structures, and only within these does the
Transcriptome analysis of sarracenia, an insectivorous plant.
Srivastava A
DNA Research : An International Journal for Rapid Publication of Reports on Genes and Genomes, 18(4), 253-261 (2011)
Asfaw Degu et al.
BMC plant biology, 19(1), 69-69 (2019-02-13)
Grape leaves provide the biochemical substrates for berry development. Thus, understanding the regulation of grapevine leaf metabolism can aid in discerning processes fundamental to fruit development and berry quality. Here, the temporal alterations in leaf metabolism in Merlot grapevine grown
Erin M Rehrig et al.
Frontiers in plant science, 5, 407-407 (2014-09-06)
Plant responses to insects and wounding involve substantial transcriptional reprogramming that integrates hormonal, metabolic, and physiological events. The ability to respond differentially to various stresses, including wounding, generally involves hormone signaling and trans-acting regulatory factors. Evidence of the importance of

Produkty

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RNA rapidly and conveniently from a variety of cellular sources, including cells and tissues from mammalian, plant and bacterial cultures.

Proste metody oczyszczania DNA/RNA wspomagają analizę genomu z różnych źródeł, zwiększając efektywność badań.

Powiązane treści

Spectrum Plant Total RNA Kit oczyszcza RNA z trudnych gatunków i tkanek za pomocą zgłoszonej do opatentowania chemii.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

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