SRP3229
PDGF-BB from mouse
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonim(y):
Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF)
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About This Item
Kod UNSPSC:
12352202
NACRES:
NA.32
Polecane produkty
pochodzenie biologiczne
mouse
rekombinowane
expressed in E. coli
Próba
≥98% (HPLC)
≥98% (SDS-PAGE)
Postać
lyophilized
siła działania
1.0-2.0 ng/mL ED50
masa cząsteczkowa
24.4 kDa
opakowanie
pkg of 10 μg
metody
cell culture | mammalian: suitable
zanieczyszczenia
<0.1 EU/μg endotoxin, tested
kolor
off-white to yellow
numer dostępu UniProt
Warunki transportu
wet ice
temp. przechowywania
−20°C
informacje o genach
mouse ... PDGFB(18591)
Opis ogólny
PDGFs (platelet-derived growth factors) are disulfide-linked dimers consisting of two 12.0-13.5kDa polypeptide chains, designated PDGF-A and PDGF-B chains. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant murine PDGF-BB is a 24.4kDa disulfide-linked homodimer of two B chains (218 total amino acids).
Zastosowanie
PDGF-BB from mouse has been used as a chemotactic factor on vascular smooth muscle cell.
Działania biochem./fizjol.
The three naturally occurring PDGFs (platelet-derived growth factors); PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Heterozygous mutation in PDGFB is associated with primary familial brain calcification. It is also associated with angiogenesis for osteogenesis. PDGFB induces migration of endothelial progenitor cells and mesenchymal stem cells.
Sekwencja
MSLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT
Postać fizyczna
Lyophilized from 10 mM Sodium Citrate, pH 4.0.
Rekonstytucja
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
PDGF-BB secreted by preosteoclasts induces angiogenesis during coupling with osteogenesis.
Xie H, et al.
Nature Medicine, 20, 1270-1278 (2014)
Arterial smooth muscle cells express platelet-derived growth factor (PDGF) A chain mRNA, secrete a PDGF-like mitogen, and bind exogenous PDGF in a phenotype- and growth state-dependent manner.
Sjolund M, et al.
The Journal of Cell Biology, 106, 403-413 (1988)
Platelets in inflammation and atherogenesis.
Gawaz M, et al.
The Journal of Clinical Investigation, 115, 3378-3384 (2005)
A role for the polysialic acid-neural cell adhesion molecule in PDGF-induced chemotaxis of oligodendrocyte precursor cells.
Zhang H, et al.
Journal of Cell Science, 117, 93-103 (2004)
William H Thiel et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 24(4), 779-787 (2016-01-07)
Inhibition of vascular smooth muscle cell (VSMC) proliferation by drug eluting stents has markedly reduced intimal hyperplasia and subsequent in-stent restenosis. However, the effects of antiproliferative drugs on endothelial cells (EC) contribute to delayed re-endothelialization and late stent thrombosis. Cell-targeted
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