SRP0237

PI3 kinase (p120g) Active human

recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)

• Synonim(y): PIK3CG, Phosphoinositide-3-kinase, catalytic, gamma, p120-PI3K
• Kod UNSPSC: 12352200
• NACRES: NA.32

Właściwości

• pochodzenie biologiczne: human
• rekombinowane: expressed in baculovirus infected insect cells
• Próba: ≥90% (SDS-PAGE)
• Postać: aqueous solution
• aktywność właściwa: ≥1.4 pmol/min-μg
• masa cząsteczkowa: 131 kDa
• opakowanie: pkg of 20 μg
• stężenie: >0.02 mg/mL
• numer dostępu NCBI: AF327656
• numer dostępu UniProt: P48736
• Warunki transportu: dry ice
• temp. przechowywania: −70°C
• informacje o genach: human ... PIK3CG(5294)

Opis

Opis ogólny

Human p120γ ( GenBank Accession No. AF327656), full length with N-terminal His tag, MW = 131 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Zastosowanie

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Definicja jednostki

One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.

Postać fizyczna

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Uwaga dotycząca przygotowania

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

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