SRP0194

PARP3 Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

• Synonim(y): ADPRT3, ADPRTL2, IRT1, NAD(+) ADP-ribosyltransferase 3, Poly (ADP-ribose) Polymerase 3
• Kod UNSPSC: 12352200
• NACRES: NA.77

Właściwości

• pochodzenie biologiczne: human
• rekombinowane: expressed in baculovirus infected insect cells
• Próba: ≥80% (SDS-PAGE)
• Postać: aqueous solution
• masa cząsteczkowa: 87 kDa
• opakowanie: pkg of 10 μg
• stężenie: >0.02 mg/mL
• numer dostępu NCBI: NM_001003931
• numer dostępu UniProt: Q9Y6F1
• Warunki transportu: dry ice
• temp. przechowywania: −70°C
• informacje o genach: human ... PARP3(10039)

Opis

Opis ogólny

Human PARP3 (GenBank Accession No. NM_001003931), full length with N-terminal GST tag, MW = 87 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Zastosowanie

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Definicja jednostki

One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute (room temperature) from NAD into acid-insoluble form.

Postać fizyczna

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Uwaga dotycząca przygotowania

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

This page may contain text that has been machine translated.