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SHCLNV

Sigma-Aldrich

MISSION® shRNA

Lentiviral Particles

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About This Item

Kod UNSPSC:
41106609

Poziom jakości

200

metody

capture ELISA: 106 VP/mL using p24

Warunki transportu

dry ice

temp. przechowywania

−70°C

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Opis ogólny

Our comprehensive shRNA product offering consists of over 150,000 pre-cloned shRNA constructs targeting more than 15,000 human and 15,000 mouse genes. The library is available in: gene family sets: gene collections related to specific functional classes such as kinases and ion channels; gene taget sets, sets of shRNA clones that target your favorite gene; and individual shRNA clones. Our shRNA and lentiviral manufacturing platforms utilize dedicated laboratories featuring state-of-the-art robotics, liquid handling and laboratory information management systems (LIMS) to provide superior quality and service for your RNAi research needs.
Lentiviral particles are ideal for transducing a wide range of cell lines. VSV-G Psuedotyping of the MISSION® lentiviral particles allows for entry into a wide range of cells. In addition, lentiviral particles stably integrate the shRNA into the genomes of both dividing and non-dividing cell lines. The MISSION bioproduction team regularly produces high quality lentivirus, so that our customers can get straight to their RNAi experiment without wasting precious time on tedious lentiviral production. Our minimum titer is 106 VP/mL. Unlike adenoviral infections that require vast excesses, lentiviral transduction is efficient enought allow for MOI as low as 1.

Zastosowanie

MISSION® shRNA has been used:
  • in transfections and transductions
  • in the establishment of stable cell lines expressing shRNA
  • in the generation of alpha-actinin-4 (ACTN4) knockdown cell line
  • to transduce raw 264.7 cells

Inne uwagi

To see protocols and application data please visit sigma.com/shrna.

Informacje prawne

Use of this product is subject to one or more license agreements.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

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Certyfikaty analizy (CoA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Dokumenty section.

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Sejal Desai et al.
Biochimica et biophysica acta, 1865(1), 196-208 (2017-10-23)
Acquired radioresistance accompanied with increased metastatic potential is a major hurdle in effective radiotherapy of breast cancers. However, the nature of their inter-dependence and the underlying mechanism remains largely intangible. By employing radioresistant (RR) cell lines, we herein demonstrate that
Xia Liu et al.
Oncology letters, 16(5), 5868-5874 (2018-10-20)
Ovarian cancer (OC) has the highest fatality rates of all gynecological malignancies worldwide. The epithelial-to-mesenchymal transition (EMT) serves an essential role in the progression of OC. An improved understanding of the molecular mechanism underlying EMT in OC may increase the
Barbara D Boyan et al.
Scientific reports, 8(1), 8588-8588 (2018-06-07)
Successful osseointegration of an endosseous implant involves migration and differentiation of mesenchymal stem cells (MSCs) on the implant surface. Micro-structured, hydrophilic titanium surfaces direct MSCs to undergo osteoblastic differentiation in vitro, in the absence of media additives commonly used in
Vera Mugoni et al.
Cell research, 29(6), 446-459 (2019-04-27)
Although targeted therapies have proven effective and even curative in human leukaemia, resistance often ensues. IDH enzymes are mutated in ~20% of human AML, with targeted therapies under clinical evaluation. We here characterize leukaemia evolution from mutant IDH2 (mIDH2)-dependence to
Inhibitors of endoplasmic reticulum alpha-glucosidases potently suppress hepatitis C virus virion assembly and release
Qu X, et al.
Antimicrobial agents and chemotherapy, 55(3), 1036-1044 (2011)
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Produkty

MISSION® Lentiviral shRNA

MISSION shRNA reduce the expression of specific target genes by targeting the specific mRNA therefore reducing the corresponding protein expression.

Safe Lentivirus Handling

Our lentiviral vector systems are developed with enhanced safety features. Numerous precautions are in place in the design of our lentiviruses to prevent replication. Good handling practices are a must.

Bezpieczna obsługa lentiwirusa

Systemy wektorów lentiwirusowych mają priorytetowe znaczenie dla bezpieczeństwa, a środki ostrożności zapobiegają replikacji. Dobre praktyki obsługi są niezbędne do użytkowania.

Successful Transduction Using Lentivirus

Successful targeting relies on optimizing key sensitive steps in the process, including lentiviral transduction. Below are some helpful handling and titration tips from our R&D lentiviral experts.

Zobacz wszystko

Protokoły

CRISPR Lentiviral Screening: Antibiotic Selection

You are not alone designing successful CRISPR, RNAi, and ORF experiments. Sigma-Aldrich was the first company to commercially offer lentivirus versions of targeted genome modification technologies and has the expertise and commitment to support new generations of scientists.

FACS Titration of Lentivirus Protocol

FACS (Fluorescence-Activated Cell Sorting) provides a method for sorting a mixed population of cells into two or more groups, one cell at a time, based on the specific light scattering and fluorescence of each cell. This method provides fast, objective, and quantitative recording of fluorescent signals from individual cells.

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