SAB4200005

Anti-UVRAG antibody ,Mouse monoclonal

clone UVRAG-11, purified from hybridoma cell culture

• Synonim(y): Anti-DHTX, Anti-UV radiation resistance-associated gene protein, Anti-p63
• Kod UNSPSC: 12352203
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• białko sprzężone: unconjugated
• forma przeciwciała: purified from hybridoma cell culture
• rodzaj przeciwciała: primary antibodies
• klon: UVRAG-11, monoclonal
• Postać: buffered aqueous solution
• masa cząsteczkowa: antigen ~90 kDa
• reaktywność gatunkowa: mouse, human
• stężenie: ~1.0 mg/mL
• metody: immunoprecipitation (IP): suitable; western blot: 2-4 μg/mL using whole extract of human G361 cells
• izotyp: IgG1
• numer dostępu UniProt: Q9P2Y5
• Warunki transportu: dry ice
• temp. przechowywania: −20°C
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... UVRAG(7405) ; mouse ... Uvrag(78610)

Opis

Opis ogólny

Monoclonal Anti-UVRAG (mouse IgG1 isotype) is derived from the hybridoma UVRAG-11 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human UVRAG.

Ultraviolet irradiation resistance-associated gene (UVRAG) is a crucial autophagic tumor suppressor, encoded by the gene mapped to human chromosome 11q13.5. The encoded protein is characterized with four functional domains, such as proline-rich domain, a lipid-binding C2 domain, a Beclin1-binding coiled-coil domain (CCD) and a C-terminal domain involved in centrosome integrity and DNA damage repair.

Zastosowanie

Monoclonal Anti-UVRAG antibody produced in mouse has been used in immunoprecipitation.

Monoclonal Anti-UVRAG antibody produced in mouse has been used in western blotting and immunohistochemistry.

Działania biochem./fizjol.

UVRAG is positive regulator of the Beclin 1- class III phosphatidylinositol 3-kinase (PI(3)KC3) complex. The tumor suppressor Beclin 1 forms a complex with PI(3)KC3, promoting autophagosome formation. UVRAG interacts with Beclin 1 through their coiled-coil domains and induces autophagy resultin in suppression of proliferation and tumorigenicity of human colon cancer cells. UVRAG is a tumor suppressor candidate.

Ultraviolet irradiation resistance-associated gene (UVRAG) is implicated in the regulation of intracellular membrane trafficking, including autophagy and chromosomal stability. The encoded protein regulates apoptosis by suppressing the BCL2-associated X protein (Bax) activity. Mutation in the gene has been observed in various types of human cancers, including microsatellite unstable colon carcinomas.

Opis wartości docelowych

Anti-UVRAG, UV radiation resistance associated gene, complements the ultraviolet sensitivity of xeroderma pigmentosum group C cells and encodes a protein with a C2 domain. The protein activates the Beclin1-PI(3)KC3 complex, promoting autophagy and suppres

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable