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R1137
Hind III from Haemophilus influenzae
Restriction Enzyme
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About This Item
Numer CAS:
Numer MDL:
Kod UNSPSC:
12352204
Polecane produkty
klasa czystości
for molecular biology
Formularz
buffered aqueous glycerol solution
stężenie
≥10000 units/mL
10,000 units/mL
Warunki transportu
wet ice
temp. przechowywania
−20°C
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Specyficzność
Recognition sequence: 5′-A/AGCTT-3′
Cutting results: 2-10-fold Hind III overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 65 °C for 15 minutes
Cutting results: 2-10-fold Hind III overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 65 °C for 15 minutes
Zastosowanie
HindIII, a restriction endonuclease, is used in molecular biology applications to cleave DNA at the recognition site 5′-A/AGCTT-3′ to generate DNA fragments with cohesive 5′-ends.
Inne uwagi
Supplied with 10x Restriction Enzyme Buffer SB (B8781)
Comment: Hind III under suboptimal reaction conditions will cleave secondary recognition sites (star activity).
Postać fizyczna
Solution in 10 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 1 mM dithioerythritol, 250 mM NaCl, 0.01% polydocanol (v/v), 50% glycerol (v/v) at 4 °C
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Numer produktu
Opis
Cennik
produkt powiązany
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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