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NA0410

Sigma-Aldrich

GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit

greener alternative

sufficient for 25 preparations

Synonim(y):

Gen Elute, GenElute Endotoxin-Free Plasmid Kit

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Wszystkie zdjęcia(2)

About This Item

Kod UNSPSC:
41105501
NACRES:
NA.52

zastosowanie

sufficient for 25 preparations

Poziom jakości

200

charakterystyka ekologicznej alternatywy

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

metody

DNA extraction: suitable

kategoria ekologicznej alternatywy

Aligned,

temp. przechowywania

15-25°C

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Opis ogólny

The GenElute HP Endotoxin-Free Plasmid Maxiprep Kit offers a simple and rapid method for isolating endotoxin-free plasmid DNA from recombinant E. coli cultures. The kit uses a vacuum format with a filter column for the rapid clearing of the bacterial lysate and a silica column for capturing plasmid DNA. A proprietary solution binds plasmid DNA to the binding column while preventing endotoxins from adsorbing. The technology allows for the user to consistently achieve levels of endotoxin that are less than 0.1 endotoxin units per μg of plasmid DNA.

High-quality, endotoxin-free DNA is ready for immediate use for the most demanding applications including transfection with endotoxin-sensitive cells.

Zastosowanie

GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit has been used in the isolation of plasmid from swine liver and from competent Escherichia coli cells.

Cechy i korzyści

  • Up to 1.2 mg of high-copy plasmid DNA with endotoxin levels of ≤0.1 EU/μg
  • Only 35 minutes from pelleted cells to purified plasmid
  • Convenient vacuum format

Zasada

An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis. The lysate is clarified by filtration followed by the addition of a binding solution that has been optimized for endotoxin-free plasmid preparations. The plasmid DNA is then captured on silica, while endotoxins are prevented from adsorbing to the membrane. Two wash steps remove contaminants. Finally, the bound DNA is eluted in endotoxin-free water. The recovered plasmid DNA is predominately in its supercoiled form. Genomic DNA and RNA are below detectable levels by ethidium bromide stained agarose gel electrophoresis.

Inne uwagi

For additional information, please see www.sigma-aldrich.com/genelutehp.

Informacje prawne

GenElute is a trademark of Sigma-Aldrich Co. LLC
This page may contain text that has been machine translated.

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

H226,H290,H302,H315,H319,H334,H336

Klasyfikacja zagrożeń

Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organy docelowe

Central nervous system

Kod klasy składowania

3 - Flammable liquids

Temperatura zapłonu (°F)

77.0 °F - closed cup

Temperatura zapłonu (°C)

25 °C - closed cup

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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Odwiedź Bibliotekę dokumentów

Successful liver-directed gene delivery by ERCP-guided hydrodynamic injection (with videos)
Kumbhari V, et al.
Gastrointestinal Endoscopy, 88(4), 755-763 (2018)
Towards a metalloprotease-DNA vaccine against piscine cryptobiosis caused by Cryptobia salmositica
Tan CW, et al.
Parasitology Research, 102(2), 265-275 (2008)
Keith Hansen et al.
Journal of visualized experiments : JoVE, (64)(64), e3304-e3304 (2012-06-27)
Genome editing is a powerful technique that can be used to elucidate gene function and the genetic basis of disease. Traditional gene editing methods such as chemical-based mutagenesis or random integration of DNA sequences confer indiscriminate genetic changes in an
Paul A Beare et al.
Journal of bacteriology, 191(5), 1369-1381 (2008-12-31)
Coxiella burnetii is a gram-negative obligate intracellular bacterium and the causative agent of human Q fever. The lack of methods to genetically manipulate C. burnetii significantly impedes the study of this organism. We describe here the cloning and characterization of
João M Furtado et al.
Investigative ophthalmology & visual science, 53(11), 6856-6862 (2012-09-07)
Toxoplasma gondii, the parasite responsible for ocular toxoplasmosis, accesses the retina from the bloodstream. We investigated the dendritic cell as a potential taxi for T. gondii tachyzoites moving across the human retinal endothelium, and examined the participation of adhesion molecules
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