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N5415
Nerve Growth Factor-ß human
≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture
Synonim(y):
β-NGF, BNGF, NGF-β, NGF-beta
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About This Item
Kod UNSPSC:
12352202
NACRES:
NA.77
Polecane produkty
Nazwa produktu
Nerve Growth Factor-ß human, recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture
pochodzenie biologiczne
human
Poziom jakości
rekombinowane
expressed in HEK 293 cells
Próba
≥95% (SDS-PAGE)
Formularz
lyophilized powder
siła działania
≤16 ng/mL ED50/EC50
masa cząsteczkowa
protein calculated mol wt 13.5 kDa (Monomer)
opakowanie
pkg of 10 μg
pkg of 100 μg
metody
cell culture | mammalian: suitable
zanieczyszczenia
≤1 EU/μg Endotoxin (β-NGF)
numer dostępu UniProt
temp. przechowywania
−20°C
informacje o genach
human ... NGF(4803)
Opis ogólny
NGF-β gene is mapped to human chromosome 1p13.2.
Recombinant human β-NGF is expressed in human 293 cells as a homodimer (non-disulfide bonded) of 120 amino acids, with a calculated molecular weight of 13.5 kDa (monomer). Nerve growth factor β (β-NGF) is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. The production of natural β-NGF occurs in epithelial cells, and the pituitary gland.
Działania biochem./fizjol.
A mutation in the NGF-β gene is implicated in the loss of pain perception.
Nerve growth factor β (β-NGF) is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. β-NGF promotes the survival and cholinergic phenotype of basal forebrain cholinergic neurons and stimulates neurite outgrowth of embryonic trigeminal motor neurons in vitro.
Przestroga
The product retains activity for at least 2 years as supplied. After initial thawing it is recommended to store the protein in working aliquots at -20 °C.
Postać fizyczna
This product is lyophilized from PBS.
Uwaga dotycząca przygotowania
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile DDW containing 0.1% endotoxin-free recombinant human serum albumin, to a final concentration of 100 μg/ml.
Przechowywanie i stabilność
Store product at –20 °C. The product retains activity for at least 2 years as supplied. After initial thawing it is recommended to store the protein in working aliquots at –20 °C.
Komentarz do analizy
The specific activity is determined by the dose dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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The neurotrophin family is comprised of the structurally related secreted proteins nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophine-4 (NT-4). They bind and activate the tyrosine kinase receptors Trk A, B, and C in a ligand-specific
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