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Key Documents
N3520
Nutrient Mixture F-12 Ham
Kaighn′s Modification, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Synonim(y):
Ham’s F-12
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About This Item
Polecane produkty
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powder
metody
cell culture | mammalian: suitable
komponenty
NaHCO3: no
sodium pyruvate: 0.22 g/L
HEPES: no
L-glutamine: yes
phenol red: yes
Warunki transportu
ambient
temp. przechowywania
2-8°C
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Opis ogólny
The nutrient mixture Ham′s F-12 is developed by Ham and it has higher levels of amino acids, vitamins and trace elements. Putrescine and linoleic acid are added to the formulation. Nutrient medium was originally designed for the serum-free growth of Chinese hamster ovary and lung cells. When used with whole or dialyzed serum or in combination with hormones and transferrin, it is widely used to grow mammalian and hybridoma cells.
Zastosowanie
Nutrient Mixture F-12 Ham is used for the following applications:
Kaighn′s modification of Ham′s F-12 and Coon′s F-12 has increased concentrations of amino acids and pyruvate, as well as modified salts (Konigsbergs). This medium was designed to support the growth of differentiated rat and chicken cells, and primary human liver cells.
- HeLa (human cervix adenocarcinoma cells) were grown in this medium (nutrient mixture F-12 Ham) supplemented with 10% heat-inactivated fetal calf serum
- Used in cell culture for different cells during maintenance and incubation (i.e. A549 cell line was maintained as a monolayer in nutrient mixture F-12 HAM Medium along with other components)
- Used in media for the propagation of Cowdria ruminantium
- Used during preparation of primary mixed glial cultures
Kaighn′s modification of Ham′s F-12 and Coon′s F-12 has increased concentrations of amino acids and pyruvate, as well as modified salts (Konigsbergs). This medium was designed to support the growth of differentiated rat and chicken cells, and primary human liver cells.
Ilość
Formulated to contain 11.1 grams of powder per liter of medium.
Rekonstytucja
Supplement with 2.5 g/L sodium bicarbonate.
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Numer produktu
Opis
Cennik
Hasło ostrzegawcze
Warning
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Skin Sens. 1
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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Protokoły
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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