M4892
McCoy′s 5A Medium
Modified, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Produkcja farmaceutyczna
Synonim(y):
5A basal medium
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About This Item
Polecane produkty
Poziom jakości
Formularz
powder
metody
cell culture | mammalian: suitable
komponenty
phenol red: 0.011 g/L
L-glutamine: 0.21915 g/L
glucose: 3.0 g/L (Dextro)
NaHCO3: no
Warunki transportu
ambient
temp. przechowywania
2-8°C
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Opis ogólny
McCoy′s 5A Medium is recommended to produce viruses in primary cell cultures.
Zastosowanie
McCoy′s 5A Medium has been used for culturing:
- human colon carcinoma cell lines (HCT116, RKO, FET, CBS, HCT116b, and TENN) to study the function of leucine-rich repeat-containing G-protein-coupled receptor (LGR5) in colon cancer metastasis
- human colorectal HCT116 cells to study miR-224 over-expression in cancer
- osteoblasts to study ion reactivity of calcium-deficient hydroxyapatite in standard cell culture media
Działania biochem./fizjol.
McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.
Ilość
Formulated to contain 11.9 grams of powder per liter of medium.
Rekonstytucja
Supplement with 2.2 g/L sodium bicarbonate.
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Numer produktu
Opis
Cennik
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
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Produkty
Zmodyfikowana formuła nośnika McCoy's 5A
Protokoły
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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