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Merck

M4892

SAFC

McCoy′s 5A Medium

Modified, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Produkcja farmaceutyczna

Synonim(y):

5A basal medium

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About This Item

Numer MDL:
Kod UNSPSC:
12352207
NACRES:
NA.71

Poziom jakości

Formularz

powder

metody

cell culture | mammalian: suitable

komponenty

phenol red: 0.011 g/L
L-glutamine: 0.21915 g/L
glucose: 3.0 g/L (Dextro)
NaHCO3: no

Warunki transportu

ambient

temp. przechowywania

2-8°C

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Opis ogólny

McCoy′s 5A Medium is recommended to produce viruses in primary cell cultures.

Zastosowanie

McCoy′s 5A Medium has been used for culturing:
  • human colon carcinoma cell lines (HCT116, RKO, FET, CBS, HCT116b, and TENN) to study the function of leucine-rich repeat-containing G-protein-coupled receptor (LGR5) in colon cancer metastasis
  • human colorectal HCT116 cells to study miR-224 over-expression in cancer
  • osteoblasts to study ion reactivity of calcium-deficient hydroxyapatite in standard cell culture media

Działania biochem./fizjol.

McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.

Ilość

Formulated to contain 11.9 grams of powder per liter of medium.

Rekonstytucja

Supplement with 2.2 g/L sodium bicarbonate.
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3


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Certyfikaty analizy (CoA)

Lot/Batch Number

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Yan Zhang et al.
Oncotarget, 8(53), 91134-91149 (2017-12-07)
Human osteosarcoma is considered a malignant tumor with poor prognosis that readily metastasizes. Tetrahydrocurcumin (THC) has been reported to have anti-tumor activity in numerous tumors. In addition, hypoxia-inducible factor-1α (HIF-1α) has been demonstrated to be associated with tumor metastasis by
Yu Wang et al.
PloS one, 8(7), e68744-e68744 (2013-08-08)
MicroRNA-224 (miR-224) is frequently over-expressed in liver and colorectal cancers. We and others have previously described the role of miR-224 over-expression in cell proliferation in vitro but we have yet to identify the relevant miR-224 direct target. In this study
Ying Liu et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 44(4), 1396-1410 (2017-12-05)
Osteosarcoma (OS) is the most common primary malignant tumor of bone tissue. Although treatment effectiveness has improved, the OS survival rate has fluctuated in recent years. Andrographolide (AG) has been reported to have antitumor activity against a variety of tumors.
Archana Kumari et al.
The EMBO journal, 39(14), e104006-e104006 (2020-06-23)
Cellular studies of filamentous actin (F-actin) processes commonly utilize fluorescent versions of toxins, peptides, and proteins that bind actin. While the choice of these markers has been largely based on availability and ease, there is a severe dearth of structural
Chuan-Ming Xie et al.
Autophagy, 10(9), 1495-1508 (2014-06-24)
EEF2K (eukaryotic elongation factor-2 kinase), also known as Ca (2+)/calmodulin-dependent protein kinase III, functions in downregulating peptide chain elongation through inactivation of EEF2 (eukaryotic translation elongation factor 2). Currently, there is a limited amount of information on the promotion of

Produkty

Zmodyfikowana formuła nośnika McCoy's 5A

Protokoły

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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