N3129

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt

phosphatase substrate, chromogenic, ≥97% anhydrous basis, powder

• Synonim(y): p-Nitrophenyl phosphate bis(cyclohexylammonium) salt, PNPP
• Wzór liniowy: O₂NC₆H₄OP(O)(O^-)₂ · 2C₆H₁₁NH₃⁺
• Numer CAS: 52483-84-8
• Masa cząsteczkowa: 417.44
• Beilstein: 6553276
• Numer WE: 257-949-0
• Numer MDL: MFCD00013173
• Kod UNSPSC: 12352204
• Identyfikator substancji w PubChem: 24897623
• NACRES: NA.83

Właściwości

• product name: 4-Nitrophenyl phosphate bis(cyclohexylammonium) salt, phosphatase substrate
• Poziom jakości: 300
• Próba: ≥97% anhydrous basis
• Postać: powder
• rozpuszczalność: water: 10 mg/mL, clear, colorless to faintly yellow
• temp. przechowywania: −20°C
• ciąg SMILES: NC1CCCCC1.NC2CCCCC2.OP(O)(=O)Oc3ccc(cc3)[N+]([O-])=O
• InChI: 1S/C6H6NO6P.2C6H13N/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*7-6-4-2-1-3-5-6/h1-4H,(H2,10,11,12);2*6H,1-5,7H2
• Klucz InChI: KXNNUYCXODNFBD-UHFFFAOYSA-N

Opis

Zastosowanie

4-Nitrophenyl phosphate bis(cyclohexylammonium) salt has been used as a component in the colorimetric reagent for abscisic acid (ABA) assay.

Działania biochem./fizjol.

p-Nitrophenyl phosphate (pNPP) is the preferred substrate for use with alkaline phosphatase in enzyme-linked immunosorbent assay (ELISA) procedures. Due to its high sensitivity, pNPP is the considered substrate in alkaline phosphatase systems. ELISA applications based on pNPP can be read in timed assays or delayed readings by stopping with alkaline solutions. This substrate yields a yellow-colored soluble end product that can be measured spectrophotometrically at 405 nm.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)