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N2770

Sigma-Aldrich

p-Nitrophenyl phosphate

SIGMAFAST, chromogenic, tablet

Synonim(y):

Tabletki pNPP

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About This Item

Numer WE:
Numer MDL:
Kod UNSPSC:
12352204
NACRES:
NA.83

Nazwa produktu

SIGMAFAST p-Nitrophenyl phosphate Tablets, tablet, To prepare 20 mL

Poziom jakości

Formularz

tablet

rozpuszczalność

water: 0.25 tablet/mL, clear, colorless

temp. przechowywania

−20°C

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Zastosowanie

Developed for use as a soluble substrate for the detection of alkaline phosphatase activity in Enzyme Immunoassays (EIA). pNPP is the substrate of choice in high sensitivity alkaline phosphatase-based EIA assays.One pNPP tablet and one Tris buffer tablet are dissolved in deionized water and provide a ready-to-use substrate. Each SIGMAFAST pNPP tablet set yields a solution containing 1.0 mg/ml pNPP, 0.2 M Tris buffer. EIA applications using pNPP may be read in timed assays or stopped with 3N NaOH for delayed readings. SIGMAFAST require only the addition of distilled or deionized water to prepare an active substrate solution. Tablets are individually packaged in foil packets.

Rekonstytucja

Each tablet set dissolved in 20 mL deionized water yields a ready-to-use buffered solution containing pNPP.

Informacje prawne

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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Certyfikaty analizy (CoA)

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Odwiedź Bibliotekę dokumentów

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Yau-Hung Chen et al.
International journal of biological macromolecules, 126, 159-169 (2018-12-27)
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G Gasperini et al.
Infection and immunity, 89(4) (2020-12-16)
Typhoid and paratyphoid fevers have a high incidence worldwide and coexist in many geographical areas, especially in low-middle-income countries (LMIC) in South and Southeast Asia. There is extensive consensus on the urgent need for better and affordable vaccines against systemic
Öykü Üzülmez et al.
Frontiers in plant science, 12, 723363-723363 (2021-10-22)
Peanut allergy is a potentially life-threatening disease that is mediated by allergen-specific immunoglobulin E (IgE) antibodies. The major peanut allergen Ara h 2, a 2S albumin seed storage protein, is one of the most dangerous and potent plant allergens. Ara
Fernando Diaz et al.
Virology, 513, 129-135 (2017-10-27)
Herpes simplex virus 2 (HSV-2) is the leading cause of genital herpes and increases the risk of HIV infection, but there is no effective vaccine. A replication-defective HSV-2 mutant virus, dl5-29, is effective in animal models and has been in

Produkty

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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