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A9544

Sigma-Aldrich

Anti-Human IgG (Fc specific)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonim(y):

Anti Human Igg, Anti-Human IgG Antibody - Anti-Human IgG (Fc specific)-Alkaline Phosphatase antibody produced in goat, Anti-Human Igg, anti human IgG antibody

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About This Item

Numer MDL:
MFCD00162437
Kod UNSPSC:
12352203
NACRES:
NA.46

pochodzenie biologiczne

goat

białko sprzężone

alkaline phosphatase conjugate

forma przeciwciała

affinity isolated antibody

rodzaj przeciwciała

secondary antibodies

klon

polyclonal

Postać

buffered aqueous solution

metody

direct ELISA: 1:55,000
dot blot: 1:40,000 (chemiluminescent)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150

Warunki transportu

wet ice

temp. przechowywania

2-8°C

docelowa modyfikacja potranslacyjna

unmodified

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Opis ogólny

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. IgG is usually found as a monomer. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. About 70 percent of the total immunoglobulin consists of IgG. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Anti-Human IgG (Fc specific)-Alkaline Phosphatase antibody is specific for human IgG and human IgG, Fc fragment. The product does not react with human IgG, Fab fragment, light chains, IgA, and IgM. Additionally, the antibody does not bind to mouse and rat IgG. Goat anti-human IgG is conjugated to alkaline phosphatase by protein cross linking with 0.2% glutaraldehyde.

Specyficzność

Specificity of the Anti-Human IgG is determined prior to conjugation by ELISA. The conjugate is specific for human IgG and human IgG, Fc fragment. Cross reactivity of the antibody-conjugate is also determined by ELISA. The conjugate shows no reactivity with human IgG, Fab fragment, human light chains, human IgA, human IgM or with mouse IgG and rat IgG.
Binds human IgG; does not bind other human Igs.
Provides reduced background with mouse or rat samples.

Immunogen

Human IgG, Fc fragment

Zastosowanie

A modified monoclonal antibody specific immobilization of platelet antigens (MAIPA) assay was performed on human platelet eluates using alkaline phosphatase-conjugated goat anti-human, Fc specific, antibody as the secondary with a 30 minute incubation.
Anti-Human IgG (Fc specific)-Alkaline Phosphatase antibody is suitable for use in ELISA and western blot at 1:5000 dilutions. The product can also be used for dot blot (1:40,000) and immunohistochemistry (1:150 using formalin-fixed, paraffin-embedded sections).

Anti-Human IgG (Fc specific)-Alkaline Phosphatase antibody has been used in
  • quantitative sandwich ELISA
  • α-GalC ELISA
  • ELISA assays
  • ELISAs for IgG subclasses

Działania biochem./fizjol.

Immunoglobulin G (IgG) regulates immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders. Immunoglobulin G (IgG) mainly participates in hypersensitivity type II and type III.

Postać fizyczna

Solution in 0.05 M Tris, pH 8.0, containing 1% BSA, 1 mM MgCl2 and 15 mM sodium azide

Przechowywanie i stabilność

Store at 2-8 °C. Do Not Freeze.

Inne uwagi

No cross-reaction with mouse and rat IgG.
Working dilutions should be determined by titration assay. Due to differences in assay systems, these titers may not reflect the user′s actual working dilution.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

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Odwiedź Bibliotekę dokumentów

Human interleukin 10 induces naive surface immunoglobulin D+ (sIgD+) B cells to secrete IgG1 and IgG3.
Briere F, et al.
The Journal of Experimental Medicine, 179(2), 757-762 (1994)
Cross-reactive phage-displayed mimotopes lead to the discovery of mimicry between HSV-1 and a brain-specific protein.
Cortese I, et al.
Journal of Neuroimmunology, 113(1), 119-128 (2001)
Julia Karbach et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 17(4), 861-870 (2010-12-18)
NY-ESO-1, one of the most immunogenic tumor antigens, is expressed in 15% to 25% of metastatic prostate cancers. The immunological and clinical effects of vaccination with recombinant NY-ESO-1 protein combined with CpG as adjuvant were evaluated. In a phase I
Long-lived plasma cells from human small intestine biopsies secrete immunoglobulins for many weeks in vitro.
Mesin L, et al.
Journal of Immunology, 1003181-1003181 (2011)
Sreenivasulu B Reddy et al.
PloS one, 7(2), e32242-e32242 (2012-03-01)
Malaria kills almost 1 million people every year, but the mechanisms behind protective immunity against the disease are still largely unknown. In this study, surface plasmon resonance technology was used to evaluate the affinity (measured as k(d)) of naturally acquired
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