MTOX1004

MDR1/BCRP Double Knockout Caco-2 Cells

Human male colorectal tissue, adenocarcinoma

• Synonim(y): C2BBe1 Cells MDR1/BCRP (-/-/-/-,-/-/-/-)
• Kod UNSPSC: 41106514
• NACRES: NA.81

Właściwości

• product name: MDR1/BCRP Double Knockout Caco-2 Cells, one vial
• pochodzenie biologiczne: human male colorectal tissue (Source disease: adenocarcinoma)
• Postać: liquid
• metody: drug transporter assay: suitable; permeability assay: suitable
• numer dostępu OMIM: 171050; 603756
• Zastosowanie: ADME/TOX
• temp. przechowywania: −196°C
• informacje o genach: human ... ABCB1(5243) , ABCG2(9429)

Opis

Opis ogólny

The C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The MDR1 and BCRP knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the ABCB1 and ABCG2 (MDR1/BCRP) efflux transporters.

Zastosowanie

The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:

Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases

Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes

Caco-2 Transporter Knockout Cell Based Assays

Cechy i korzyści

The Caco-2 subclone C2BBe1 cells are ideal for transporter analysis as they express multiple transporters, are human derived, and grow in a homogenous monolayer that forms tight junctions necessary for efflux ratio analysis. Other benefits include:

• A functional double knockout of the MDR1 gene and BCRP gene eliminates the reliance on chemical inhibitors to determine if a compound is an MRP2 substrate
• The vial format enables the MDR1 and BCRP knockout cells to be included in standard drug transporter protocols
• Human assay with no interference from animal inhibitors
• Overcome the limitations of RNAi and knockdown cell lines that arise from remaining transporter functionality

Informacje prawne

ADME/Tox Cell Lines License

Oświadczenie o zrzeczeniu się odpowiedzialności

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

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Elementy zestawu są też dostępne oddzielnie

• MTOX1004 MDR1/BCRP Double Knockout Caco-2 Cells, one vial 1 vial

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable