L6007

Leucine Aminopeptidase, microsomal from porcine kidney

Type VI-S, lyophilized powder, ≥12 units/mg protein (biuret)

• Synonim(y): Aminopeptidase M, Aminopeptidase N, Cathepsin III, LAP, Leucinamide aminopeptidase, Leucinaminopeptidase
• Numer CAS: 9054-63-1
• Numer EC enzymu: 3.4.11.2 (BRENDA, IUBMB)
• Numer WE: 232-942-5
• Numer MDL: MFCD00131501
• Kod UNSPSC: 12352204
• NACRES: NA.54

Właściwości

• pochodzenie biologiczne: Porcine kidney
• typ: Type VI-S
• Postać: lyophilized powder
• aktywność właściwa: ≥12 units/mg protein (biuret)
• masa cząsteczkowa: 320 kDa
• skład: Protein, 30-70%
• temp. przechowywania: −20°C

Opis

Opis ogólny

Leucine Aminopeptidase (LAP) from porcine is a 320-kDa exopeptidase present in cytosol. It contains zinc in its structure and belongs to the M1 and M17 peptidase families. LAP display bi-lobed structure and has the catalytic domain at the C-terminus and a variable N-terminal domain.

Zastosowanie

Leucine Aminopeptidase, microsomal from porcine kidney has been used in aminopeptidase N (APN) inhibitor screening assay in adenocarcinoma cell line ES-2 and in the inhibition kinetic studies with APN inhibitor.

Działania biochem./fizjol.

Leucine Aminopeptidase (LAP) catalyzes protein hydrolysis at the leucine residue at N-terminal. An altered level of LAP is implicated in tumor proliferation and progression. LAP is a potential biomarker for ovarian epithelial cancer therapy. Porcine LAP hydrolyzes glycine and arginine substrates.

Definicja jednostki

One unit will hydrolyze 1.0 μmole of L-leucine-p-nitroanilide to L-leucine and p-nitroaniline per min at pH 7.2 at 37 °C. (At 25 °C, approx. 40% of the activity at 37 °C is obtained.) The activity obtained using L-leucine p-nitroanilide as substrate is 2-5 times that obtained with L-leucinamide as substrate.

Postać fizyczna

Contains primarily phosphate buffer salts, pH 7.0

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08,GHS07
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H315,H319,H334,H335
• Zwroty wskazujące środki ostrożności: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Klasyfikacja zagrożeń: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organy docelowe: Respiratory system
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)